围着床期小鼠子宫中巨噬细胞的变化及作用  

Role of macrophages in mouse uterine during the peri-implantation period

作　　者：高瑞花[1] 陈雷宁[1] 谭雯雅[1] 谭洪川[1] 欧湘红[1] 李红[1] 全松[1] 

机构地区：[1]南方医科大学南方医院妇产科生殖医学中心,广东广州510515

出　　处：《南方医科大学学报》2015年第3期365-369,共5页Journal of Southern Medical University

基　　金：国家自然科学基金(81170575);广东省自然科学基金(9151051501000066)~~

摘　　要：目的观察小鼠子宫中胚胎着床部位与非着床部位巨噬细胞(Macrophage,Mφ)的分布差异,探讨其在胚胎着床过程中的作用。方法取真孕(n=30)和假孕小鼠(n=30)子宫组织,选择30只妊娠小鼠模型作为实验组,同时选择30只假孕小鼠模型作为对照组,在围着床期取两组小鼠子宫组织,采用免疫组化技术检测子宫组织中Mφ、诱导型一氧化氮合成酶(i NOS)、白血病抑制因子(LIF)的分布,分析比较子宫组织中胚胎着床部位和非着床部位Mφ、i NOS和LIF的分布差异及相关性。结果在小鼠子宫组织中,观察到实验组Mφ在D4.5(雌鼠交配后次日晨阴道见栓为D0.5)前主要分布子宫内膜,D4.5后主要分布于子宫外膜,对照组Mφ主要分布于子宫内膜;实验组i NOS在D4.5前主要分布子宫内膜,D4.5后主要见于子宫肌膜和外膜,对照组i NOS主要位于子宫内膜,且实验组Mφ和i NOS分布呈正相关。此外,实验组LIF显著表达于子宫内膜,对照组几乎不表达,二者间有统计学差异(P<0.05);着床部位Mφ、i NOS和LIF明显高于非着床部位,其中Mφ、LIF比较有统计学差异(P<0.05)。结论围着床期小鼠子宫组织中Mφ主要分布在子宫内膜及胚胎着床点,并伴有i NOS和LIF的表达,提示Mφ在胚胎着床中可能发挥重要作用。Objective To investigate the effect of macrophages on embryo implantation by observing the distribution of macrophages in mouse uterine tissues during the peri- implantation period. Methods Uterine tissues were collected from pregnant（n=30） and pseudopregnant mice（n=30） during the peri- implantation period. The distributions of macrophages,i NOS and leukemia inhibitory factor（LIF） were determined by immunohistochemistry and the correlations of macrophages with i NOS and LIF were analyzed. Results Macrophages were located mainly in the endometrium before D4.5 in the pregnant rats with D0.5 defined as the morning when a vaginal plug was observed. After D4.5, the macrophages was significantly reduced in number（P〈0.05） in the endometrium and gradually migrated to the perimetrium. In the psudopregnant mice,macrophages were located mainly in the endometrium. Before D4.5, i NOS- positive cells were detected mainly in the endometrium and the myometrium in the pregnant rats and became significantly reduced on D4.5（P〈0.05）; in the pseudopregnant mice, the positive cells were mostly detected in the endometrium. Significant differences were found in the distribution of the macrophages and LIF between the implantation and non- implantation sites（P=0.013）. LIF was mostly located in the endometrium in the pregnant mice but scarcely detected in the pseudopregnant mice. Conclusion Macrophages are located mainly in the endometrium and the implantation site where i NOS and LIF are expressed, suggesting the important role of macrophages in the determination of implantation.

关 键 词：围着床期 子宫 巨噬细胞 白血病抑制因子 诱导型一氧化氮合成酶 

分 类 号：R714.8[医药卫生—妇产科学]