丰富环境及反转缝合治疗后突触素对关键期内视皮质神经元可塑性的影响  

Effects of synaptophysin on plasticity of visual cortex neurons after rich environment and reverse suture treatment in critical period

作　　者：蔺静静[1] 刘向玲[2] 王圆月[3] 宋子宣[2] 李靳[4] 张锐[1] 

机构地区：[1]新乡医学院,河南省新乡市453003 [2]新乡医学院第三附属医院眼科,河南省新乡市453003 [3]漯河市中心医院,河南省漯河市462000 [4]泰山医学院附属医院,山东省泰安市271000

出　　处：《眼科新进展》2015年第4期330-333,共4页Recent Advances in Ophthalmology

基　　金：河南省教育厅2011年医学科技攻关项目(编号:011A320012)~~

摘　　要：目的探讨关键期内单眼形觉剥夺性弱视大鼠模型经反转缝合及丰富环境治疗前后视皮质突触素(synaptophysin,SYP)的表达规律,及SYP对视皮质神经元可塑性的影响。方法 36只14 d龄SD大鼠随机分为6组,每组6只,其中NCI组、NCII组为正常对照组;MDI组、MDII组、反转缝合(reverse suture,RS)组和RS+丰富环境(enriched environment,EE)组分别行右眼眼睑缝合,RS组、RS+EE组于28 d龄打开剥夺眼,同时缝合对侧眼睑行遮盖治疗,RS组放于标准环境中饲养、RS+EE组放于EE中饲养;NCI组、MDI组于28 d龄,NCII组、MDII组、RS组、RS+EE组于42 d龄行图形视觉诱发电位检测,同时采用HE染色和免疫组织化学法检测分析各组大鼠视皮质SYP蛋白表达水平的变化。结果图形视觉诱发电位示形觉剥夺眼P100波的波形稳定性差、潜伏期延长、振幅降低。HE染色示各组大鼠视皮质神经元均未见明显异常。免疫组织化学法检测可见RS组视皮质SYP阳性神经元密度较MDII组高,RS+EE组视皮质SYP阳性神经元密度较MDII组、RS组高,MDI组大鼠视皮质SYP阳性神经元密度较NCI组低,MDII组、RS组大鼠视皮质SYP阳性神经元密度均较NCII组及RS+EE组低,定量分析显示以上各组SYP免疫标记的平均光密度值间差异均有统计学意义(均为P<0.05),但NCII组和RS+EE组两组间平均光密度值差异无统计学意义(P>0.05)。结论 SYP影响视皮质神经元可塑性,且参与了视觉发育视皮质可塑性的变化过程,是弱视发病的重要分子机制之一;Objective To investigate the expression of synaptophysin（ SYP） and its effects on plasticity of neurous in the visual cortex of monocularly deprived rats before and after reverse suture combined with enriched environment treatment in the critical period. Methods Thirtysix14-day-old rats were randomly divided into six groups,6 cases in each group,in which normal control group I（ NCI group） and normal control group II（ NCII group） were the normal control group,the right eyelid of rats in monocular deprivation group I（ MDI group）,monocular deprivation group II（ MDII group）,reverse suture group（ RS group） and RS ＋ enriched environment group（ RS ＋ EE group） were sutured,the eyelid of 28-day-old rats in RS group and RS ＋ EE group were opened,then the other side eyelid were sutured. The rats in RS group were bred in the normal environment,and RS ＋ EE group bred in EE. The pattern visual evoked potential of 28-day-old rats in NCI group and MDI group,42-day-old rats in NCII group,MDII group,RS group and RS ＋ EE group were measured,and the expression of SYP protein in the visual cortex of the different groups were detected by immunohistochemistry staining and HE staining. Results The pattern visual evoked potential showed that the stability of P100 wave in the deprived eye was poor,the latency was increased,and the amplitude was decreased.The HE staining showed that the visual cortex neurons of rats in each group unchanged.The immunohistochemical staining showed that： The density of positive neurons of SYP in the visual cortex of RS group was significantly higher than those in MDII group,RS ＋ EE group was higher than MDII group and RS group,MDII group was lower than NCI group,MDII group and RS group were lower than MDII group and RS ＋ EE group; The quantitative analysis showed that,except for NCII group and RS ＋ EE group,there were statistical differences in density of positive neurons between each other two groups（ all P〈0. 05）. Conclusion SYP affect the plasticity of ne

关 键 词：单眼形觉剥夺 弱视 突触素 视皮质 反转缝合治疗 丰富环境 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]