盐酸小檗碱慢性毒性实验  

The chronic toxicity experiment of Berberine in rat

作　　者：张洪超[1,2] 谭伟[1] 褚晴晴[1] 张萍[3] 王涛[1] 马廷贤[1] 王雪梅[1] 池志宏[1] 

机构地区：[1]中国医科大学病理生理教研室,辽宁沈阳110122 [2]黑龙江省医院普外科,黑龙江哈尔滨150030 [3]辽宁医学院组织胚胎学教研室,辽宁锦州121001

出　　处：《解剖科学进展》2015年第2期159-162,共4页Progress of Anatomical Sciences

基　　金：东北制药集团公司资助横向课题;国家自然科学基金资助项目(No.81170561);辽宁省科技计划项目(No.2013225090;No.2012408002)

摘　　要：目的研究小檗碱的慢性毒性反应。方法在急性毒性试验中,给予大鼠不同小檗碱剂量测试小檗碱的半数致死量(LD50)和最大耐受剂量。慢性毒性试验中,给予大鼠0.25,0.5,1g/(kg·d)连续给药12周,然后观察大鼠生长发育、血液学、生化、脏器指数和脏器形态学变化。结果急性毒性试验中,大鼠给予最大灌胃剂量3000mg/kg后未见大鼠死亡,半数致死量和最大耐受量未检出。慢性毒性试验中,与对照组大鼠相比,除中、高剂量组大鼠存在稀便现象外,各剂量组大鼠在行为学,血液学、生化、脏器指数等方面均无显著差异。结论小檗碱对大鼠没有明显的急慢性毒性,可连续安全口服。Objective The purpose of this paper is to study the chronic toxicity of Berberine. Methods In the acute toxicity test, rats were given different doses of berberine to test the LD50 and maximum tolerated dose. For the chronic toxicity test, rats were given 0.25, 0.5, 1g /（kg·d） Berberine administered continuously for 12 weeks, and then the growth and development of rats, hematology, biochemistry, organ coefficients and morphological changes of organs were observed. Results In acute toxicity test, no rats were found death after given a maximum perfusion gastric dose 3000mg/kg Berberine, with no half lethal dose and the maximum tolerance being detected. For the chronic toxicity test, compared with the control rats, in addition to 0.5g/kg and 1g/kg groups rats with loose bowels phenomenon, there was no significant difference in ethology, hematology, biochemistry, organ coefficients for each dose group of rat. Conclusion Berberine has no acute toxicity and chronic toxicity, and no potential adverse for rats for long-time use.

关 键 词：小檗碱 急性毒性试验 慢性毒性试验 大鼠 

分 类 号：R965.3[医药卫生—药理学]