人MTERF4基因cDNA的克隆及编码蛋白质的生物信息学分析  

Cloning and bioinformatic analysis of mitochondrial transcription termination factor 4(MTERF4) cDNA in Homo sapiens

作　　者：熊伟[1,2] 杨勇琴[1] 张海洋[1] 徐彤[1] 张晓娟 余敏[4] 

机构地区：[1]大理学院基础医学院,云南大理671000 [2]云南省昆虫生物医药研发重点实验室,云南大理671000 [3]大理市第一人民医院,云南大理671000 [4]云南大学生命科学学院,云南昆明650091

出　　处：《生物技术》2015年第1期28-35,共8页Biotechnology

基　　金：大理学院博士科研启动基金项目(No.BSKY2012018);云南省教育厅科学研究重点项目(No.2014Z126);云南省大学生创新创业计划项目(No.201408)资助~~

摘　　要：[目的]克隆人线粒体转录终止因子4（mitochondrial transcription termination factor 4,MTERF4）基因cDNA编码区,预测和分析其编码蛋白质的结构与功能。[方法]采用RT-PCR技术扩增人MTERF4基因cDNA的编码序列,采用生物信息学方法对人MTERF4蛋白进行分析。[结果]人MTERF4基因cDNA编码序列为1 146bp,编码一个由381氨基酸组成的亲水性蛋白质,分子量为45.78 k D,理论等电点为9.49。该蛋白N端1-42个氨基酸为前导肽序列,定位于细胞线粒体。其二级结构主要为α螺旋和无规则卷曲,包含4个MTERF基序。三级结构预测结果与二级结构相符。蛋白质多重序列比对和聚类分析显示,人MTERF4蛋白与大鼠、小鼠的MTERF4蛋白具有高度同源性,在系统发育树上聚为一类。[结论]人MTERF4基因cDNA的克隆及编码蛋白质的生物信息学分析为进一步研究其生物学功能奠定了理论依据。[Objective]Human mitochondrial transcription termination factor 4（MTERF4） gene was cloned and analyzed the structure and functional characteristics of human MTERF4 by bioinformatics method. [Methods]RT-PCR was used to cloned the cDNA coding sequence of human MTERF4,many bioinformatic methods were used to analysis its structure and function. [Results]The results showed that,the ORF of human MTERF4 cDNA was 1 146 bp,encode the hydrophilic protein consist of 381 amino acids. The calculated molecular mass of human MTERF4 was 45. 78 k D. The theoretical isoelectric point was 9. 49. Human MTERF4 localized in mitochodria,and the N-termination 1-42 aa were transit peptide. Main composition of the protein secondary structure were（-helix and random coil,it contains four MTERF motifs. The multiple alignment and clustering analysis showed that,human MTERF4 is a orthologous protein belong to MTERF family,and have high similarity to Rattus norvegicus and Mus musculus MTERF4. [Conclusion]The cloning of human MTERF4 and bioinformatic analysis results provide bioinformation which will helpful for the further study of the biological function of h MTERF4.

关 键 词：人线粒体转录终止因子4 CDNA克隆 结构 功能 生物信息学 

分 类 号：Q811.4[生物学—生物工程]