非诺贝特对大鼠颅脑损伤后核转录因子、白细胞介素－6和细胞凋亡的影响  

Influence of fenofibrate on tissue NF-KB, IL-6 and cell apoptosis in secondary brain injury after traumatic brain injury in rats

作　　者：孙长忠[1] 王永青[2] 彭余江[3] 邵波[3] 余智 

机构地区：[1]浙江中医药大学附属嘉兴市中医院神经外科,浙江省嘉兴314001 [2]复旦大学附属上海华山医院神经外科 [3]浙江大学附属第二医院神经外科 [4]浙江省淳安县第一人民医院神经内科

出　　处：《中国医师杂志》2015年第3期372-375,共4页Journal of Chinese Physician

基　　金：嘉兴市科技局基金资助项目（2012JXCB0019）

摘　　要：目的：探讨非诺贝特干预对大鼠颅脑损伤后脑组织中NF－κB p65、IL－6和细胞凋亡的变化及其可能机制。方法98只健康雄性SD大鼠按随机数字表法分为非诺贝特组（ n ＝49）及对照组（ n ＝49），前者通过改良Feeney法建立颅脑损伤模型后立即予以非诺贝特60 mg／（kg·d）灌胃，对照组则建立模型后予生理盐水2 ml／（ kg·d）灌胃，以后各组分别每24小时灌胃一次等量非诺贝特／生理盐水，直至动物被处死，根据伤后处死时间分为1、3、6、12、24 h、3 d和7 d共7个亚组，每亚组各7只。取损伤灶周围组织采用免疫组织化学方法检测并比较挫伤灶周围脑组织中NF－κB p65及IL－6蛋白表达情况，同时采用TUNEL法观察并比较脑挫伤灶周围细胞凋亡情况。结果非诺贝特组NF－κB p65及IL－6蛋白表达水平较对照组均明显下降（ P ＜0．05），且两组中二者均呈正相关（ P ＜0．01）；同时非诺贝特组脑组织细胞凋亡数量较对照组也有所下降（ P ＜0．05）。结论颅脑损伤后非诺贝特可能通过降低NF－κB p65活性，控制炎症反应，减少细胞凋亡，从而对颅脑损伤后的神经细胞发挥保护作用。Objective To investigate the changes of NF-κB p65, IL-6 and cell apoptosis in sec-ondary brain injury after traumatic brain injury and the influence of fenofibrate on these parameters in rats. Methods Ninety-eight male Sprague-Dawley （ SD） rats were randomly divided into two groups：fenofibrate group （ n =49） and control group （ n =49） .The fenofibrate group was induced with the improved Feeney method and received intragastrica of lipanthyl 60 mg/（kg·d） immediately after injury.The control group were received intragastrica of sodium chloride injection 2 ml/（ kg·d） immediately after injury and twice everyday until rats were killed.Each group was divided into seven subgroups by sacrificed time after injury, those were 1 h, 3 h, 6 h, 12 h, 24 h, 3 d, and 7 d, and each subgroup got 7 rats.Each subgroup was ran-domly selected three rats after being killed to detect expressions of NF-κB p65 and IL-6 of rat contusion peri tissues brain tissues with immunohistochemical method.While using terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling （ TUNEL） method was used to observe the peri cell apoptosis after brain contusion.Results The expressions of NF-κB p65 and the IL-6 in each fenofibrate group were significantly decreased relative to the control group （ P 〈0.05）,and a significant positive correlation between both pa-rameters in two groups （ P 〈0.01） .At the same time, the number of apoptotic cells was decreased （ P 〈0.05）.Conclusions Fenofibrate was probably through the route of relieving inflammation response to re-duce the change of secondary brain injury after traumatic brain injury and decrease neural cell apoptosis, and then provide protection of neurocytes.

关 键 词：普鲁脂芬/药理学 颅脑损伤/药物疗法/代谢/病理学 NF-κB/代谢 白细胞介素6/代谢 细胞凋亡 

分 类 号：R651.15[医药卫生—外科学]