CD31在带部分松质骨的小牛皮质骨板移植愈合中的表达研究  

Study on CD31 expression in the process of the calf cortical with partial cancellous bone xenograft

作　　者：禹晓东 刘傥[2] 张湘生[2] 罗令 雷霆[3] 

机构地区：[1]长沙市中医医院长沙市第八医院骨二科,长沙410100 [2]中南大学湘雅二医院骨科 [3]湖南省儿童医院骨科

出　　处：《中国医师杂志》2015年第3期387-390,共4页Journal of Chinese Physician

摘　　要：目的：观察CD31在带部分松质骨的小牛皮质骨板移植愈合过程中不同时相的表达及组织分布情况，探讨其愈合机制及再血管化情况与骨改建的关系，为进一步研究组织工程骨奠定实验基础。方法实验动物选用健康3月龄36只新西兰大白兔。植入带部分松质骨的小牛皮质骨骨板，分别于术后4、8、12、24周各时间点取材，通过一般情况及大体标本、X线检查、组织学及CD31免疫组织化学染色观察其愈合情况，并借助计算机图像分析仪进行定量分析，分别用再血管化指数与新骨形成面积代表再血管化和骨改建程度。结果⑴X线显示：术后24周见明显愈合。⑵在移植骨愈合的不同阶段，CD31的表达存在不同。术后4周、8周，移植骨周围的软组织、结合部、周围的骨痂及软骨巢周围见较多强阳性表达；松质骨内较多阳性表达，内见单个、条索状、团簇状新生血管，移植骨内部扩大的哈弗氏管及周围见阳性表达；术后24周，移植骨内扩大的哈弗氏管及周围呈强阳性表达，新生血管继续增多。⑶对新骨形成面积和再血管化指数的计量资料进行相关性分析表明，再血管化与骨改建呈高度正相关（ r ＝0．984）。结论 CD31的阳性表达贯穿于异种骨愈合的整个过程，而且不同时段、不同部位的CD31表达是不同的，并可以反映移植骨的再血管化与骨改建状况。骨移植后再血管化程度与骨改建密切相关。Objective To investigate the expression and tissue distribution of CD31 in the process of the calf cortical with partial cancellous bone xenograft, to explore the healing mechanism and relationship between bone remodeling and revascularization, and to provide a basis for the further study of tissue engi-neered bone.Methods Thirty-six New Zealand rabbits were selected as our experimental animals.All ani-mals were implanted with the calf cortical with partial cancellous bone.Specimens were procured at 4, 8, 12 and 24 weeks after surgery, respectively.The expression and tissue distribution of CD31 were observed in the process healing with general condition, X-ray, histology and immunohistochemistry.The image analy-sis computer system was used for quantitative analysis.The extent of revascularization and remodeling was separately represented by revascularization indexes and new bone areas.The relationship was studied be-tween bone remodeling and revascularization.Results ⑴ The X-ray showed significant healing.⑵ The positive expression of CD31 was seen in the whole process of bone xenograft and was different at various stage and sites.By the 4 weeks and 8 weeks postoperative,the strong positive expression was seen in the pe-ripheral soft tissue of graft bone, the callus of joint and the periphery of cartilage nests.Much positive ex-pression was seen in the cancellous bone.There was seen some single, cord, and cluster vascular endotheli-al cells.The positive expression was seen in the enlarged haversian canals and periphery.By the 24 weeks postoperative,the positive expression was seen in the enlarged haversian canals and periphery with increasing newborn capillaries.⑶ The correlation analysis between new bone areas and revascularization showed a closely positive correlation （ r =0.984）.Conclusions The positive expression of CD31 was seen in the whole process of bone xenograft and was different at various stage and sites.The positive expression of CD31 reflected the extent of revascularization and bone remode

关 键 词：抗原 CD31/代谢 骨移植 骨折愈合 骨板 

分 类 号：R687.3[医药卫生—骨科学]