p53基因表达对低出生体质量大鼠肾脏功能的影响  

Effect of p53-gene expression on renal function in rat with low birth weight

作　　者：盖建芳[1] 冀湧[1] 马荣伟[1] 姚建宏[1] 

机构地区：[1]山西省儿童医院NICU,太原030013

出　　处：《中国医师杂志》2015年第3期398-402,共5页Journal of Chinese Physician

摘　　要：目的：研究p53基因表达对低出生体质量（ LBW）大鼠肾功能的影响。方法将15只孕鼠按随机数字表法分为正常组（5只）、LBW组（5只）和干预组（5只）。正常组母鼠全程以普通饲料（含21％蛋白）喂养；LBW组和干预组大鼠孕期均采用低蛋白饲料（含10％蛋白）喂养。仔鼠出生后母鼠均用普通饲料喂养；干预组母鼠在普通饲料喂养的基础上，于部分饮水中每日添加左旋精氨酸（ L－Arg）200 mg／kg。取7、21 d、2月龄和3月龄仔鼠标本，采用电镜观察肾小球系膜细胞和足细胞超微结构；采用RT－PCR法检查肾脏p53 mRNA的表达水平；留取血、尿标本检测大鼠肾脏功能。结果⑴肾功能比较：3月龄时，LBW组大鼠血尿素氮（ BUN）和尿肌酐（ UCr）和正常组比较差异均有统计学意义（ P ＜0．01）；2月龄时Ccr和正常组相比，LBW组显著降低（ P ＜0．05）；3月龄时LBW组和干预组大鼠Ccr均显著降低（ P ＜0．05）；与正常组相比，3月龄时LBW组和干预组大鼠24 h尿蛋白定量显著增加（ P ＜0．01， P ＜0．05）。⑵3月龄时，与正常组比较，LBW组p53 mRNA表达显著增加；3月龄LBW大鼠p53 mRNA的表达水平与Ccr水平呈负相关（ r ＝－0．91， P ＜0．05）。⑶电镜发现：LBW组肾小球系膜细胞增生伴基质增多、滤过膜足突减少及部分融合；干预组系膜细胞增生较LBW组显著减少。结论 p53基因在LBW大鼠肾组织呈过度表达，LBW大鼠肾功能减退，采用L－Arg干预LBW大鼠肾脏功能有一定程度的改善，提示p53基因参与了LBW大鼠肾功能的损害，也是肾功能减退的原因之一。Objective To investigate the effect of p53-gene expression on renal function in rat with low birth weight （ LBW） .Methods Fifteen pregnant rats were randomly assigned into normal group, LBW group, and L-arginine （ L-Arg）-treated group.For normal group, pregnant rats were fed with 21% protein diet during pregnancy.For LBW and L-Arg treated groups, rats were fed with 10%protein diet.After de-livery, all rats were fed with 21%protein diet.For L-Arg-treated group, rats were given a supplementation with L-Arg （200 mg/kg·drinking water during 21 d lactation, other rats were given running water.At the age of 2m, the ultrastructural change in mesangial cell and podocyte was observed with electron microscope. At the age of 7 d, 21 d, 2 m, and 3 m old, the p53 mRNA expression in renal tissues was observed with re-verse transcription polymerase chain reaction （ RT-PCR） , blood and urine were collected to detect biochem-ical indicators of renal function and 24 h-urine protein.Results ⑴At the age of 3 m,the blood urea nitro-gen （ BUN） and urine cretinine （ UCr） in normal group were significantly lower than those in LBW group （ P 〈0.01）.Compared to normal group, the Ucr of LBW group was significantly lower at the age of 2 m （ P〈0.05）.At the age of 3 m,the Ucr of L-Arg treated group and LBW group was significantly lower （ P 〈0.05）, the level of 24 h-Urine protein was notably increased in LBW group and L-Arg treated group than that in normal group （ P 〈0.01, P 〈0.05） .⑵At the age of 3 m,the expression of p53 mRNA in LBW group was higher obviously than that in normal group.There is a significantly negative correlation between＆amp;nbsp;the expression of p53 mRNA and the level of Ucr in LBW group （ r =-0.91, P 〈0.05）.⑶ There were mesangial cell proliferation with matrix increase, filtration membrane podocyte reduction, and partially dis-solved in LBW group, the mesangial cell proliferation of L-Arg treated group was decreased compared to that in LBW group.Conclusions Th

关 键 词：基因 P53 出生体质量 肾/代谢/病理学 GENES P53 

分 类 号：R3416[医药卫生—基础医学]