纳米羟基磷灰石/壳聚糖/半水硫酸钙为可注射骨组织工程支架材料的可行性  

Feasibility of nano-hydroxyapatite/chitosan/calcium sulphate hemihydrate as an injectable bone tissue engineering scaffold

作　　者：薛震[1] 牛丽媛[2] 安刚[3] 郭亚山[3] 吕松岑[1] 

机构地区：[1]哈尔滨医科大学附属第二医院骨科四病房,黑龙江省哈尔滨市150086 [2]哈尔滨工业大学校医院,黑龙江省哈尔滨市150086 [3]哈尔滨医科大学附属第二医院急诊创伤病房,黑龙江省哈尔滨市150086

出　　处：《中国组织工程研究》2015年第8期1160-1164,共5页Chinese Journal of Tissue Engineering Research

基　　金：黑龙江省教育厅科学技术研究项目(12511202)~~

摘　　要：背景:前期实验采用仿生学原理制备了可注射性纳米羟基磷灰石/壳聚糖/半水硫酸钙复合材料,但其与骨髓间充质干细胞的生物相容性还不十分清楚。目的:探讨纳米羟基磷灰石/壳聚糖/半水硫酸钙作为注射型骨组织工程支架材料的可行性。方法:将第3代兔骨髓间充质干细胞与可注射纳米羟基磷灰石/壳聚糖/半水硫酸钙支架复合培养,作为实验组;以单纯接种培养的骨髓间充质干细胞为对照组,倒置显微镜下观察细胞生长情况,MTT法检测细胞增殖,扫描电镜观察细胞在材料表面生长与增殖。将纳米羟基磷灰石/壳聚糖/半水硫酸钙支架埋植在家兔背部肌袋内,埋植后2,4,6,8周进行病理学观察。结果与结论:实验组细胞生长、增殖良好,与对照组无明显差异。支架埋植后2周,材料周围有中等量中性粒细胞、淋巴细胞和巨细胞浸润,可见小血管与纤维母细胞增生,材料已被炎性细胞分割、围绕散碎;埋植后4周,可见少量淋巴细胞、纤维母细胞聚集,炎症反应进一步消退,肌纤维排列、形态正常;埋植后6周,材料周围炎症反应轻微,组织水肿不明显;埋植后8周,炎症反应基本消退,材料基本降解完成,肌纤维形态基本正常。表明纳米羟基磷灰石/壳聚糖/半水硫酸钙复合物具有良好的细胞相容性和生物降解性,可作为注射型支架材料。BACKGROUND：In preliminary experiments, injectable nano-hydroxyapatite/chitosan/calcium sulphate hemihydrates （nHA/CS/CSH） composites have been developed based on the bionics principle, but the biocompatibility of this composite with bone marrow mesenchymal stem cels is not very clear. OBJECTIVE：To study the feasibility of nHA/CS/CSH as an injectable bone tissue engineering scaffold. METHODS： Bone marrow mesenchymal stem cels from rabbits were cultured and induced, and the third passage cels were cultured with nHA/CS/CSH as an experimental group. Bone marrow mesenchymal stem cels subjected to inoculated culture were used as controls. The morphology and growth of the cels were observed under inverted phase contrast microscope. MTT assay was used to examine cel proliferation. The morphology and proliferation of the cels on the surface of nHA/CS/CSH was observed by scanning electron microscope. The nHA/CS/CSH scaffold was implanted into the rabbit sacrospinal muscles, and pathologicaly observed after 2, 4, 6, 8 weeks. RESULTS AND CONCLUSION：Bone marrow mesenchymal stem cels co-cultured with nHA/CS/CSH exhibited good growth as observed under inverted phase contrast microscope, without significant difference from those in the control group. At 2 weeks after scaffold implantation, a moderate amount of neutrophils, lymphocytes and giant cels infiltrated around the graft material, smal blood vessels and fibroblasts proliferated, and the graft material was divided and embraced by inflammatory cels; at 4 weeks after implantation, a few lymphocytes, fibroblasts aggregated, the inflammation subsided further, and muscle fibers showed normal arrangement and morphology; at 6 weeks after implantation, the inflammation around the graft material became mild and tissue edema was not obvious; at 8 weeks after implantation, the inflammation subsided basicaly, the material degradation was complete, and the muscle fibers had normal morphology. These findings indicate that the nHA/CS/CSH composite material has good biocompa

关 键 词：生物材料 骨生物材料 半水硫酸钙 组织工程 羟基磷灰石 壳聚糖 生物相容性 骨髓间充质干细胞 

分 类 号：R318[医药卫生—生物医学工程]