骨髓间充质干细胞与磷酸钙骨水泥复合物修复兔关节软骨缺损  

Bone marrow mesenchymal stem cells combined with calcium phosphate cement to repair articular cartilage defects in rabbits

作　　者：周常艳[1] 周庆焕[2] 边竟[1] 陈克[1] 陈文[1] 

机构地区：[1]十堰市太和医院(湖北医药学院附属医院)骨科一病区,湖北省十堰市442000 [2]湖北医学院护理学院,湖北省十堰市442000

出　　处：《中国组织工程研究》2015年第8期1195-1199,共5页Chinese Journal of Tissue Engineering Research

摘　　要：背景:前期实验已成功将骨髓间充质干细胞接种于磷酸钙骨水泥支架,并证实其具有良好的机械强度和生物相容性。目的:观察新西兰大白兔骨髓间充质干细胞体外培养后与磷酸钙骨水泥复合修复关节软骨缺损的可行性。方法:选取18只新西兰兔用电钻制成股骨滑车部5 mm的骨-软骨缺损模型,随机选择15只兔于骨缺损处左侧植入单纯磷酸钙骨水泥材料作为对照组,于右侧植入骨髓间充质干细胞与磷酸钙骨水泥复合物作为实验组,另3只兔不植入任何材料作为空白组。分别于4,8,16周各时间点处死兔取材,进行X射线摄片、组织形态学观察及生物力学检测。结果与结论:术后4,8,16周各组骨缺损均有不同程度的骨再生,实验组新骨形成的速度和数量均优于其他组,组织学观察到实验组的成骨细胞及骨小梁出现均早于其他组。术后16周实验组骨标本抗弯曲能力的最大负荷、最大应力和破坏能量均明显高于对照组(P<0.05)。结果表明骨髓间充质干细胞复合磷酸钙骨水泥材料修复骨缺损可促进骨组织再生,恢复骨的刚度和强度,有望作为一种新型人工骨材料。BACKGROUND：In previous experiments, bone marrow mesenchymal cels have been successfuly seeded onto the calcium phosphate cement scaffold, which is confirmed to have good mechanical strength and biocompatibility. OBJECTIVE： To observe the feasibility of rabbit bone marrow mesenchymal stem cels combined with calcium phosphate cements embedded in articular cartilage defects. METHODS：Eighteen New Zealand rabbits were used to create 5 mm bone-cartilage defects on both sides of the mandible, and then, 15 rabbits were randomly selected and implanted with simple calcium phosphate cements into the left bone defects as control group and with bone marrow mesenchymal stem cels combined with calcium phosphate cement into the right bone defects as experimental group. Another three rabbits were implanted with nothing as blank group. After transplantation 4, 8, 16 weeks, rabbits from each group were sacrificed. Clinical observation, X-ray imaging, histological observation were performed at different periods. RESULTS AND CONCLUSION：After 4,8,16 weeks, bone tissue regeneration in the experimental group was better than the other two groups in the bone formation speed and quantity. The maturity of new bone tissue, scaffold degradation, bone maturation in the experimental group was better than that in the other two groups. After 16 weeks, the maximum load, maximum stress and damage energy of the bone specimens in the experimental group were significantly higher than those in the control group. These findings indicate that bone marrow mesenchymal stem cels combined with calcium phosphate cement can promote bone regeneration, restore bone stiffness and strength, which is expected to act as a new artificial bone material in repairing bone defects.

关 键 词：生物材料 软骨生物材料 磷酸钙骨水泥 骨髓间充质干细胞 骨缺损 生物力学 骨再生 

分 类 号：R318[医药卫生—生物医学工程]