腺苷酸环化酶Ⅲ(AC3)在非神经细胞内的定位（英文）  

Subcellular Localization of Adenylate Cyclase Ⅲ in Non-Neuronal Cells

作　　者：郝江叶[1] 韩绍芳 罗影涛 杨栋[1] 李超[1] 张 曹振龙[1] 胡远想 刘伟丽[2] 倪志华[1] 周艳芬[1] 王振山[1] 

机构地区：[1]河北大学生命科学学院,保定071002 [2]河北大学基础医学院,保定071002

出　　处：《中国细胞生物学学报》2015年第3期378-386,共9页Chinese Journal of Cell Biology

基　　金：supported by the National Natural Science Foundation of China(Grant No.31171191);the Natural Science Foundation of Hebei Province(Grant No.C2012201106);the Scientific Research Foundation for the Returned Overseas Chinese Scholars,State Education Ministry of China(<2013>693);the Introducing Talent Foundation of Hebei University(Grant No.2011-215)~~

摘　　要：腺苷酸环化酶III(adenylate cyclase III,AC3)最早发现于小鼠主要嗅觉表皮,是c AMP信号通路的组成成分之一,它的缺失可导致小鼠嗅觉丧失、体重增加、雄性不育、母性关爱行为丧失和学习与记忆能力下降等。脊椎动物体内绝大多数细胞具有纤毛,在小鼠大脑神经元中AC3定位于初级纤毛,许多非神经类型细胞也有AC3的表达,然而其与非神经类细胞纤毛的关系尚待确定。该文选择了8种细胞株(2种神经类细胞株、6种非神经类细胞株),对上述问题进行了研究。RT-PCR及其产物DNA测序结果显示,这些细胞中均有AC3的表达。免疫荧光染色结果显示,在IMCD3、293T和BMSCs等细胞株中,AC3与细胞初级纤毛的荧光信号重叠;在A549和He La两种癌细胞株中,AC3也定位在初级纤毛上。该研究结果表明,AC3在多数非神经细胞中也表达于纤毛部位。Adenylate cyclase Ⅲ（AC3）, which was initially discovered in the main olfactory epithelium of mouse, is an important component of the cyclic adenosine monophosphate signaling pathway. Deletion of AC3 leads to olfactory loss, weight gain, male sterility, loss of maternal care behaviors, and declines in learning and memory abilities in mice. The majority of vertebrate cells have cilia, and AC3 is localized to the cilia of neurons in the mouse brain. Although many types of non-neuronal cells also express AC3, whether it is localized in the ciliary region of non-neuronal cells is yet to be determined. In this study, eight cell lines（two neuronal cells and six nonneuronal cells） were selected to investigate the localization of AC3 in non-neuronal cells. Semi-quantitative reverse transcription-polymerase chain reaction（RT-PCR） and DNA sequencing indicated that AC3 was expressed in all of the test cells. Immunofluorescence staining showed that fluorescence signal of AC3 overlapped with that of cilia in the non-neuronal cell lines including IMCD3, 293 T and bone marrow stromal cells. Additionally, AC3 was localized to the cilia of both A549 and He La cancer cells. These data indicate that AC3 is expressed in the ciliary region in non-neuronal cells.

关 键 词：腺苷酸环化酶 初级纤毛 非神经细胞 免疫荧光染色 癌细胞 

分 类 号：Q26[生物学—细胞生物学]