结核分枝杆菌早期分泌抗原对人单核细胞活化T细胞核因子5表达的影响  

The effect of MTB antigens on the NFAT5 expression in monocytes from human peripheral blood

作　　者：刘艳华 王若 程小星 

机构地区：[1]解放军第三○九医院全军结核病研究所全军结核病防治重点实验室结核病诊疗新技术北京市重点实验室,北京100091

出　　处：《中华结核和呼吸杂志》2015年第4期290-293,共4页Chinese Journal of Tuberculosis and Respiratory Diseases

基　　金：国家自然科学基金青年基金（81101219）

摘　　要：目的 研究MTB早期分泌靶抗原6/培养滤液蛋白10（ESAT-6/CFP-10）抗原对人单核细胞的活化T细胞核因子5（NFAT5）表达的影响.方法 收集解放军第三○九医院收治的活动性肺结核患者15例,男9例,女6例,年龄33 ～42岁,平均37岁.健康人来自解放军第三○九医院健康体检中心,共15名,男9名,女6名,年龄37 ～ 42岁,平均40岁.抽取患者和健康体检者的外周血,分离单核细胞,分别用H37Rv全菌裂解液和ESAT-6/CFP-10抗原多肽刺激,提取细胞总RNA,荧光定量PCR方法检测NFAT5 mRNA的表达,采用配对t检验分析刺激前后单核细胞NFAT5mRNA的表达差异.结果 活动性结核患者刺激前单核细胞NFAT5 mRNA的相对表达量为（0.23±0.15）,EAST-6/CFP-10抗原多肽刺激后为（0.18±0.12）,表达显著下调（t=2.591,P＜0.05）,而全菌裂解液刺激后表达量（0.28±0.25）没有显著变化（t=1.142,P＞0.05）.健康人刺激前单核细胞NFAT5 mRNA的相对表达量为（0.21 ±0.09）,EAST-6/CFP-10抗原多肽刺激后为（0.17±0.09）,表达显著下调（t=2.828,P＜0.05）,而全菌裂解液刺激后表达量（0.20±0.12）没有显著变化（t=0.452,P＞0.05）.结论 MTB分泌的ESAT-6/CFP-10抗原多肽能够抑制人单核细胞中NFAT5的表达.Objective To study the effect of Mycobacterium tuberculosis antigens early secretory antigen target 6/culture filtrate protein 10 （ESAT-6/CFP-10） on nuclear factor of activated T-cells 5 （NFAT5） mRNA in monocytes from human peripheral blood.Methods Fifteen patients with active pulmonary tuberculosis （age range：33-42 y,mean：37 y,male/female：9/6） were recruited from the Institute of Tuberculosis in 309 Hospital,and 15 healthy subjects （age range：37-42 y,mean：40 y,male/female：9/6） were recruited from Medical Examination Center of the hospital.Monocytes were isolated from peripheral blood and stimulated with whole cell lysate of H37Rv strain and peptide pool of ESAT-6/CFP-10.Fluorescence quantitative PCR was used to detect the mRNA expression of NFAT5,and differences of NFAT5 mRNA between unstimulated and stimulated monocytes were analyzed by paired t test statistic method.Results The expression of NFAT5 mRNA in monocytes from patients with active pulmonary tuberculosis was significantly decreased when stimulated with peptide pool of ESAT-6/CFP-10 compared with unstimulated monocytes （0.18 ±0.12 vs.0.23 ±0.15） （t =2.591,P 〈0.05）.Similarly,the expression of NFAT5 mRNA in monocytes from healthy subjects was also significantly decreased when stimulated with peptide pool of ESAT-6/CFP-10 compared with unstimulated monocytes （0.17 ±0.09 vs 0.21 ±0.09） （t =2.828,P 〈 0.05）.However,the expression of NFAT5 mRNA in monocytes stimulated with whole cell lysate was not significantly changed （t =1.142,P 〉0.05）,which was 0.28 ±0.25 and 0.23 ±0.15 in stimulated and unstimulated monocytes,respectively.Similarly,the expression of NFAT5 mRNA in monocytes from healthy subjects was also not significantly changed when stimulated with whole cell lysate （t =0.451 9,P 〉0.05）,which was 0.20 ± 0.12 and 0.21 ± 0.09 in stimulated and unstimulated monocytes,respectively.Conclusion The MTB antigen ESAT-6/CFP-10 can inhibit NFAT5 expression in human monocytes in vitro.

关 键 词：分枝杆菌 结核 抗原 单核细胞 活化T细胞核因子5 

分 类 号：R378.911[医药卫生—病原生物学]