2型糖尿病大鼠肝脏肝细胞核因子-4α表达的变化  

CHANGES OF HEPATOCYTE NUCLEAR FACTOR 4 ALPHA EXPRESSION IN LIVER OF TYPE 2 DIABETES MELLITUS RATS

作　　者：刘东慧[1] 侯丽娜[1] 乔志燕[2] 陈志宏[1] 

机构地区：[1]承德医学院人体解剖学教研室,河北承德067000 [2]承德医学院附属医院

出　　处：《承德医学院学报》2015年第2期100-102,共3页Journal of Chengde Medical University

基　　金：国家自然科学基金项目(81441133);河北省自然科学基金项目(H2013406096);河北省高校重点发展学科"人体解剖与组织胚胎学"建设项目资助

摘　　要：目的:探讨2型糖尿病大鼠肝脏肝细胞核因子-4α(HNF-4α)表达的变化和意义。方法:24只雄性SD大鼠随机分为正常对照组和糖尿病模型组,每组12只大鼠。链脲佐菌素连续腹腔注射法制作2型糖尿病大鼠模型。采用SP免疫组织化学染色、Western Blotting法、RT-PCR法检测大鼠肝脏HNF-4α的表达情况。结果:与正常对照组大鼠比较,糖尿病模型组大鼠肝脏HNF-4α蛋白和m RNA的表达均明显升高(P<0.01)。结论:肝脏HNF-4α表达的升高可能参与了2型糖尿病时肝脏损伤的发生发展。Objective：To investigate changes and signifi cance of hepatocyte nuclear factor 4 alpha（HNF-4α） in liver of type 2 diabetes mellitus rats model.Methods：24 male SD rats were randomly divided into normal control group and diabetes model group with 12 rats in each group. The type 2diabetes model rats were induced by continuous intraperitoneal injection of streptozotocin. SP immunohistochemical stain, Western Blotting and RT-PCR were used to detect HNF-4α expression in liver. Results： Compared with rats in normal control group, the HNF-4α protein and m RNA expression in liver of rats in diabetes model group increased obviously（P〈0.01）. Conclusions： High expression of HNF-4α may participate development of liver injury during diabetes mellitus.

关 键 词：2型糖尿病 肝脏 肝细胞核因子-4α(HNF-4α) 

分 类 号：R587.1[医药卫生—内分泌]