HZ-100型臭氧灭菌机对实验动物屏障设施熏蒸灭菌效果观察  

Observation on the Effect of Sterilization by Ozone Fumigation System HZ-100 Used for Barrier Facilities for Laboratory Animals

作　　者：范涛[1] 邢进[1] 李保文[1] 

机构地区：[1]中国食品药品检定研究院实验动物资源研究所,北京100050

出　　处：《中国药事》2015年第3期298-302,共5页Chinese Pharmaceutical Affairs

基　　金：实验动物质量评价方法及其标准化研究与应用(编号2013BAK11B02)

摘　　要：目的:对日本产HZ-100型臭氧熏蒸灭菌机在屏障环境内杀菌效果进行验证,为屏障环境内控制微生物污染提供参考。方法:使用日本产HZ-100型臭氧熏蒸灭菌机对密闭屏障房间进行熏蒸消毒处理,通过熏蒸前、后房间内菌落检测比较,观察其灭菌以及使用效果。结果:熏蒸前,房间内落下菌>59CFU/皿,墙壁拭子菌落1 CFU/皿,枯草杆菌试纸培养阳性;熏蒸后,房间内落下菌2.5 CFU/皿,墙壁拭子菌落未检出,枯草杆菌试纸培养结果阴性。结论:HZ-100型臭氧机熏蒸灭菌效果良好,全程自动化,操作便捷,使用安全。Objective： To verify the sterilization effect of the Ozone Fumigation System HZ-100 produced in Japan in the barrier environment and provide a reference for bacterial infection control in barrier facilities. Methods： The Ozone Fumigation System HZ-100 produced in Japan was used for fumigation of the hermetic barrier room. The efficacy of sterilization was evaluated by comparing bacterial colony before and after fumigation. Results： Before fumigation, the falling bacteria count was 〉59 CFU/plate, the wall swab bacteria count was 1 CFU/plate, and BI culture was positive. After fumigation, the falling bacteria count of the room was 2.5 CFU/plate, the wall swab bacteria count was zero and BI culture was negative. Conclusion： The HZ-100 Ozone machine is effective, automatic, easy to operate and safe.

关 键 词：臭氧灭菌机 实验动物环境 屏障设施 消毒处理方法 微生物污染控制 

分 类 号：R917[医药卫生—药物分析学]