实验动物标准颞骨切片规范  

Standardization of Temporal Bone Sections in Experimental Animals

作　　者：丁大连[1,2,3,5] 于进涛[1,2] 李鹏[2,3] 高可雷 蒋海燕 张文娟[4] 孙虹[1,2] 殷善开[5] Richard Salvi 

机构地区：[1]中南大学湘雅医院耳鼻咽喉头颈外科,湖南410018 [2]Center for Hearing and Deafness,Department of Communicative Disorders and Sciences,University of New York at Buffalo Buffalo NY14214 [3]中山大学附属第三医院耳鼻咽喉头颈外科,广州510630 [4]华中科技大学附属协和医院耳鼻咽喉头颈外科,武汉430022 [5]上海交通大学附属第六人民医院耳鼻咽喉头颈外科,上海200233

出　　处：《中华耳科学杂志》2015年第1期1-6,共6页Chinese Journal of Otology

摘　　要：颞骨切片技术是观察和评估实验性内耳病理学改变的重要手段。由于内耳复杂的迷宫式立体结构,从不同方向和角度获取的颞骨切片很难进行相互间的组织病理学比较。因此,统一颞骨标准切片技术规范,对于评判内耳听觉感受器和前庭各个平衡感受器以及耳蜗和前庭周边神经元的病变部位和病变程度十分重要,因为只有统一了规范的颞骨样品制备和切片角度,才有可能对实验性内耳病变做出正确的比较和评判。颞骨切片的标准方向是将切片平面平行于外耳道和内听道的水平面,从上鼓室向下鼓室方向依次收集到的需要进行重点评估的切片层次分别为上半规管壶腹嵴和外半规管壶腹嵴、椭圆囊斑和球囊斑及前庭上神经元、球囊斑和前庭下神经元、耳蜗中轴、内淋巴管及内淋巴囊、以及后半规管壶腹嵴。本文还根据作者的实践经验讨论了颞骨切片样品制备过程中有关样品固定、脱钙、颞骨整体染色、渗透和定向包埋等技术细节,期望能对从事内耳病理学研究的同道有所帮助。Preparation of the temporal hone for light microscopy is an important step in histological studies of the inner ear. Due to the complexity of labyrinthine three-dimensional structures in the inner ear, it is difficult to measure or compare structures of interest without a commonly accepted standardized measure of temporal hone sections. Therefore, standardization of temporal bone sections is very important for histological assessment of sensory hair cells and peripheral ganglion neurons in the cochlear and vestibular systems. The standardized temporal bone sectioning is oriented to achieve a horizontal plane of sec- tioning. Horizontal sections are collected from the epitympanum to the hypotympanum including layers in the order of the erista ampullaris of the superior and lateral semicircular canals, macula utriculi and macula sacculi, superior vestibular ganglion neu- rons, nlacula of saccule and inferior vestibular ganglion neurons, cochlear modiolus, endolymphatic duct and endolymphatie sac, and finally the erista ampullaris of posterior semicircular canal. Moreover, the technical details of preparing for temporal bone sectioning including fixation, decalcification, whole temporal bone staining, embedding penetration, and embedding orien- tation are also discussed.

关 键 词：实验动物标准 颞骨切片 

分 类 号：R764.3[医药卫生—耳鼻咽喉科]