噬菌体MS2和f_2对消毒剂二溴海因抵抗力研究  

Study on the resistance of bacteriophage MS2 and f_2 to dibromohydantoin disinfectant

作　　者：刘晓娟[1] 李虹霖[1] 陈昭斌[1,2] 

机构地区：[1]四川大学华西公共卫生学院,四川成都610041 [2]深圳市南山区疾病预防控制中心

出　　处：《中国消毒学杂志》2015年第4期323-325,328,共4页Chinese Journal of Disinfection

基　　金：中华预防医学会科研资金("消毒领域科研创新"专项资金)支持项目(2013DX001)

摘　　要：目的研究噬菌体MS2和f2对消毒剂二溴海因的抵抗力,探讨肠道病毒灭活效果的指示病毒。方法采用病毒悬液定量灭活试验方法,比较两种噬菌体对二溴海因的抗力。结果浓度为250 mg/L二溴海因消毒液作用5 min和10 min,对MS2噬菌体的灭活对数值分别为3.87和4.36。相同浓度的二溴海因消毒液作用5 min和10 min,对f2噬菌体的灭活对数值为3.25和3.48。二溴海因对f2噬菌体灭活对数值达到4.0以上,需要提高浓度到375 mg/L,且需要作用10 min。结论研究证明,f2噬菌体对二溴海因抗力明显高于MS2噬菌体。Objective To study the resistance of bacteriophage MS2 and f2 to dibromohydantoin disinfectant and explore the bacteriophage as virus indictor for evaluation on disinfection efficacy of enterovirus. Methods Suspension viral inactivation test was used to compare the resistance of bacteriophage MS2 and f2 to dibromohydantoin（ DBDMH）. Results With250 mg / L DBDMH for a contact time of 5 min and 10 min,the LIV of bacteriophage MS2 were 3. 87 lg and 4. 36 lg respectively. At the same condition,the LIV of bacteriophage f2 were 3. 25 lg and 3. 48 lg respectively. When 375 mg / L DBDMH for 10 min,the LIV of bacteriophage f2 could achieve the disinfection level（ LIV≥4. 00 lg）. Conclusion The resistance of the bacteriophages f2 to DBDMH is higher than bacteriophage MS2.

关 键 词：MS2噬菌体 F2噬菌体 二溴海因 抵抗力 

分 类 号：R187.1[医药卫生—流行病学]