姜黄素对大鼠重症急性胰腺炎肾脏iNOS表达的影响及其保护机理研究  

Effects of Curcumin on Expression of iNOS and Investigation The Protective Effects on Rats with Severe Acute Panceratitis Associated Renal Injury

作　　者：李之令[1,2] 张东[1] 刘江伟[1] 王皓[1] 李瑞[1] 李建英[1] 

机构地区：[1]中国人民解放军兰州军区乌鲁木齐总医院实验动物研究所新疆特殊环境医学重点实验室,新疆乌鲁木齐830000 [2]四川省凉山州第二人民医院普外科,四川西昌615000

出　　处：《中国普外基础与临床杂志》2015年第4期412-417,共6页Chinese Journal of Bases and Clinics In General Surgery

基　　金：中国博士后科学基金(项目编号:20100481517)~~

摘　　要：目的探讨姜黄素对大鼠重症急性胰腺炎肾脏诱生型一氧化氮合酶(inducible nitric oxide synthase,i NOS)m RNA表达的影响和相关性肾损伤的保护作用及其机理。方法将健康SD大鼠24只随机均分为对照组、重症胰腺炎相关性肾损伤组(简称损伤组)和姜黄素治疗组(简称治疗组),对照组和损伤组大鼠在造模前3 h分别予以1.5 m L的生理盐水灌胃,而治疗组大鼠于造模前3 h则予以1.5 m L姜黄素(200 mg/kg)生理盐水稀释液灌胃。损伤组和治疗组采用胰头夹闭法建立重症急性胰腺炎模型,造模后12 h,对照组和损伤组大鼠予以1.5 m L生理盐水灌胃,治疗组则予以1.5 m L姜黄素生理盐水稀释液灌胃。于造模后18 h采集大鼠下腔静脉血检测其血清淀粉酶、肌酐、尿素氮水平,同时采用酶联免疫吸附试验(ELISA)检测血清IL-1β、IL-6和IL-10含量;取左肾组织行病理组织学观察及采用TUNEL染色法检测肾脏细胞凋亡情况;取右肾组织采用实时-定量聚合酶链反应(RT-PCR)检测i NOS m RNA的表达。结果损伤组和治疗组大鼠血清淀粉酶、肌酐和尿素氮浓度,IL-1β、IL-6和IL-10水平,肾脏细胞凋亡指数,以及i NOS m RNA相对表达量均显著高于对照组(P<0.05),而治疗组的上述指标除IL-10水平高于损伤组(P<0.05)外,其余指标均低于损伤组(P<0.05)。结论姜黄素对大鼠重症急性胰腺炎相关性肾损伤有较好的治疗作用;其作用机理可能与姜黄素通过抑制促炎性因子IL-1β及IL-6的表达,促进抗炎性因子IL-10的表达,下调i NOS m RNA的表达,以减少氧自由基的生成和NO对细胞的损害,从而减少肾脏的细胞凋亡及坏死有关。Objective To investigate the effects of curcumin on expression of i NOS m RNA, study the protective and therapeutic effects on rats with severe acute panceratitis associated renal injury, and explore the possible mechanisms. Methods A total of 24 rats were randomly divided into control group（n=8）, severe acute panceratitis with renal injury group（injury group, n=8）, and curcumin-treated group（treated group, n=8）. The rats of control group and injury group were given 1.5 m L saline through intragastric administration at 3 h before operation, while the rats of treated group were fed with same amount of curcumin diluent at 3 h before operation. The rats of injury group and treated group with pancreatic head clamping method to establish the model of severe acute pancreatitis. At 12 h after modeling, rats in control group and injury group were perfused with 1.5 m L saline, and rats in treated group were intragastrically administrated to the same volume of curcumin diluent. All the rats were sacrificed at 18 h after modeling. The levels of serum amylase, creatinine, and blood urea nitrogen were detected and pathological chang of pancreas and the left kidney were observed under light microscope. The renal cell apoptosis were analyzed using TUNEL staining in three groups. The serum levels of interleukin-1β（IL-1β）, interleukin-6（IL-6）, and interleukin-10（IL-10） in three groups were detected by enzyme-linked immunosorbert assay（ELISA）. The expressions of inducible nitric oxide synthase（i NOS） m RNA in the right kidney were detected with realtime polymerase chain reaction（RT-PCR） in three groups. Results Compared with the control group, the levels of serum amylase, creatinine, blood urea nitrogen, IL-1β, IL-6, and IL-10, the cell apoptosis and the expressions of i NOS m RNA in injury group and treated group were significantly increased（P〈0.05）. In the treated group, the level of IL-10 was higher than the injury group（P〈0.05）, and the difference of other indexes were lower t

关 键 词：重症急性胰腺炎 肾损伤 姜黄素 诱生型一氧化氮合酶 大鼠 

分 类 号：R285.5[医药卫生—中药学]