生物素夹心法对小分子蛋白质胰岛素样生长因子1在自组装短肽水凝胶中的缓释  

Controlled Release of Low Molecular Protein Insulin-like Growth Factor-1 through Self-Assembling Peptide Hydrogel with Biotin Sandwich Approach

作　　者：刘燕飞[1] 范振海[1] 王钰莹[1] 余丽梅[1] 

机构地区：[1]遵义医学院附属医院贵州省细胞工程重点实验室,遵义563003

出　　处：《生物医学工程学杂志》2015年第2期387-392,共6页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(31360232);贵州省联合基金资助项目(黔科合J字LKZ[2013]01号)

摘　　要：蛋白质在自组装短肽水凝胶中的缓释速度与蛋白质大小和凝胶孔径直接相关,如何提高小分子蛋白质的缓释效果一直是个问题。本研究采用生物素夹心法研究胰岛素样生长因子1(IGF-1)在N端带有RGD序列的自组装短肽(P3)水凝胶中的释放效果。紫外分光光度计检测每个时间点所释放的IGF-1浓度,结果显示IGF-1的释放速度明显降低。水凝胶培养实验表明用生物素夹心法延长IGF-1释放时间能够有效地促进小鼠成软骨细胞ATDC5的增殖。Since the release rate of protein in hydrogels is directly dependent upon the size of the protein and the hydrogel,how to deliver low molecular weight protein for prolonged periods has always been a problem.In this article,we present a usage of self-assembling peptide（P3）with the RGD epitope on its N terminus.The concentration of the released insulin-like growth factor 1（IGF-1）was determined by UV-vis spectroscopy and the release kinetics suggested a notable reduction of the IGF-1release rate.Cell entrapment experiments revealed that IGF-1delivery by biotinylated nanofibers could promote the proliferation of the mouse chondrogenic ATDC5 cells when compared with cells embedded within nanofibers with untethered IGF-1.

关 键 词：生物素夹心法 自组装短肽水凝胶 蛋白质缓释 胰岛素样生长因子1 

分 类 号：Q51[生物学—生物化学]