原癌基因pim-2分子生物学特性研究  

Research on Molecular Biological Characteristics of Proto-oncogene pim-2

作　　者：周小东[1] 沈富兵[1] 

机构地区：[1]成都医学院检验医学院,成都610500

出　　处：《生物医学工程学杂志》2015年第2期405-411,共7页Journal of Biomedical Engineering

基　　金：成都医学院校基金资助项目(YCZ11-022);四川省卫生厅资助项目(120499)

摘　　要：研究原癌基因pim-2的分子生物学特性,分析其相关机制。使用生物信息学方法和技术研究分析原癌基因pim-2,用在线服务器分析pim-2基因的染色体定位,预测外显子、开放式阅读框、CpG岛和miRNAs互补片段等。用生物信息学软件预测原癌基因pim-2转录蛋白的理化性质和蛋白序列各类修饰位点,如泛素化、糖基化等,计算抗原指数,模建空间结构。结果显示原癌基因pim-2有6个外显子,CDS编码框转录一段含311个氨基酸的肽链;基因启动子存在CpG岛;3′UTR区域含miRNA基因。Pim-2蛋白分子量34 188.47,等电点5.78,不稳定指数是45.87,消光系数为279nm;蛋白序列分布着多处共价修饰位点、2处泛素化位点、4处糖基化位点、1处SUMO化位点、1处亚硝基化位点以及2处棕榈酰化位点;蛋白序列存在16段抗原指数较高区域。研究表明原癌基因pim-2序列上所分布的相关区域和修饰位点与其致癌作用存在密切关系。The purpose of this paper is to present the research on the molecular biological characteristics of proto-oncogene pim-2and to analyze the related mechanism.Proto-oncogene pim-2was studied and analyzed by the bioinformatics method and technology.With an online server,the chromosomal localization of pim-2gene was analyzed,and the exon,open reading frame,CpG island and miRNAs complementary fragments and the like were predicted.With bioinformatics software,the physicochemical property of transcription protein of proto-oncogene pim-2and various modification sites of protein sequence,such as ubiquitination and glycosylation,were predicted,the antigenic index was calculated,and the spatial structural was modeled.The research findings showed that the proto-oncogene pim-2comprised six exons,the CDS（coding sequence）transcribed a section of peptide chain including 311 amino acids,a gene promoter has a CpG island,and the 3′UTR region contains an miRNA gene.The molecular weight of the Pim-2protein was 34,188.47,the isoelectric point was 5.78,the instability index was 45.87,and the extinction coefficient was 279 nm.A plurality of covalent modification sites,two ubiquitination sites,four glycosylation sites,an SUMO sumoylation site,a nitrosation site,two palmitoylation sites and sixteen regions with higher antigenic index were distributed in the protein sequence.This research showed that the related regions and modification sites distributed on the sequence of proto-oncogene pim-2were closely related to the carcinogenic effect thereof.

关 键 词：原癌基因pim-2 生物信息学 蛋白质修饰 

分 类 号：R346[医药卫生—基础医学]