血清Ang/Tie2、VEGF在糖尿病肾病病情评估中的应用价值  

Application value of serum Ang/Tie2,VEGF in the assessment of diabetic nephropathy

作　　者：霍亚杰 温玉洁[2] 刘陶文[2] 夏含笑 

机构地区：[1]桂林医学院研究生学院,桂林541001 [2]广西壮族自治区南溪山医院内分泌科,桂林541002

出　　处：《安徽医科大学学报》2015年第4期508-511,共4页Acta Universitatis Medicinalis Anhui

基　　金：广西自然科学基金资助项目(编号:2011GXNSFA018238)

摘　　要：目的观察血清中血管生成素-1(Ang-1)、血管生成素-2(Ang-2)及其受体Tie2与血管内皮生长因子(VEGF)在糖尿病肾病(DN)病情进展中的水平变化规律,探讨平衡其比率在DN中的重要性。方法选取2型糖尿病患者60例,依据尿白蛋白排泄率(UAER)分为3组:正常白蛋白尿患者20例(DM1组)、微量白蛋白尿患者22例(DM2组)、大量白蛋白尿患者18例(DM3组),另设健康对照患者22例(NC组)。采用ELISA法检测受试者血清Ang-1、Ang-2、Tie2、VEGF水平,同时检测相关生化指标。结果 1 DM1组血清VEGF、Ang-2水平高于NC组,DM2组、DM3组进一步升高,4组间差异均有统计学意义(P<0.05)。2 DM1组血清Ang-1水平高于NC组,DM2组、DM3组逐渐下降,4组间差异无统计学意义。3 Tie2水平差异无统计学意义。4UAER与Ang-2、VEGF呈正相关性(r=0.470、0.404,P<0.01),与Ang-1呈负相关性(r=-0.310,P<0.05);Ang-2与VEGF、Tie2呈正相关性(r=0.450、0.725,P<0.01);Ang-1与Tie2呈正相关性(r=0.571,P<0.01),与Ang-2、VEGF呈负相关性(r=-0.497、-0.396,P<0.01,P<0.05)。5以UAER为应变量行多元线性逐步回归分析显示:Ang-1、Ang-2、VEGF、肌酐进入回归方程,是影响UAER的独立相关因子。结论血清中Ang-1、Ang-2、Tie2、VEGF水平与DN密切相关,可能参与了DN的发生发展,并影响DN病情的进程。检测血清Ang/Tie2及VEGF可作为评估DN病情的重要指标。Objective To observe the regular levels change of serum angiopoietin-1 （Ang-1）,angiopoietin-2 （Ang-2） and receptor Tie2 as well as vascular endothelia growth factor （VEGF） in the progerssion of diabetic nephropa-thy （ DN） , and investigate the importance of ratio of balance among them in DN. Methods Sixty Chinese patients with type 2 diabetes were enrolled. According to their UAER diabetic patients were divided into three groups ran-domly：20 patients with normoalbuminuric diabetic （ DM1 group） , 22 with microalbuminuric diabetic（ DM2 group） and 18 proteinuric diabetic （DM3 group）. A total of 22 health contros were selected（NC group）. Serum Ang-1, Ang-2,Tie2,VEGF levels were detected by ELISA. At the same time, related biochemical indicators were tested. Results ①The level of serum Ang-2,VEGF increased in DM1 compared with NC, and serum Ang-2,VEGF levels in DM2 and DM3 were much higher. There was statistical difference among four groups （P〈0. 05）. ②The level of serum Ang-1 increased in DM1 compared with NC, but serum Ang-1 levels in DM2 and DM3 were much lower.③ There was no statistical significance in difference among the four groups. There was no statistical significance in differences of Tie2 .④ UAER had positive correlation with serum Ang-2 , VEGF （ r =0. 470 , 0. 404 , P 〈0. 01 ） . UAER had negative correlation with serum Ang-1（r= -0. 310,P〈0. 05）. Ang-2 had positive correlation with ser-um VEGF,Tie2（r =0. 450,0. 725,P 〈0. 01）. Ang-1 had positive correlation with serum Tie2（r =0. 571,P 〈0. 01）. Ang-1 had negative correlation with serum Ang-2,VEGF （r= -0. 497,-0. 396,P〈0. 01,P〈0. 05）. ⑤Line to UAER level as the dependent variable, multiple stepwise regression analysis showed that： Ang-1,Ang-2, VEGF,creatinine entered regression equation. They were independent correlation factors for UAER. Conclusion Serum Ang-1,Ang-2,Tie2 and VEGF levels are closely related with DN. They may participate in the development of DN and affect the p

关 键 词：糖尿病肾病 血管生成素 血管内皮生长因子 TIE2 尿白蛋白排泄率 

分 类 号：R587.2[医药卫生—内分泌]