叶酸对体外氧化损伤血管内皮细胞作用的剂量效应研究  

The dose-effect relationship of folic acid on human umbilical vein endothelial cells in vitro

作　　者：高玉霞[1] 崔杉杉[2] 李文[2] 王鹏燕[2] 肖艳宇[2] 黄国伟[2] 

机构地区：[1]天津医科大学总医院心内科,300052 [2]天津医科大学公共卫生学院营养与食品卫生学系

出　　处：《天津医药》2015年第4期337-339,I0001,共4页Tianjin Medical Journal

基　　金：国家自然科学基金资助项目(81373002)

摘　　要：目的探讨叶酸对氧化低密度脂蛋白(ox-LDL)损伤的人脐静脉内皮细胞(HUVEC)的保护作用。方法正常HUVEC按叶酸剂量不同分为叶酸缺乏组(FA-def)、FA-15、FA-50、FA-100、FA-200、FA-500 nmol/L组。正常HUVEC培养液中加入ox-LDL使其终浓度为120 mg/L,造成氧化损伤,并与7个不同叶酸浓度(0、15、60、150、225、300、375 nmol/L)共同作用24 h后,维持叶酸浓度不变更换无ox-LDL培养液继续作用72 h,并于24、48、72、96 h后收集氧化损伤细胞。采用倒置显微镜观察其形态改变,MTT法检测细胞活力改变。结果不同浓度叶酸(0、15、50、100、200、500 nmol/L)对正常HUVEC无明显促进或抑制作用(P>0.05);叶酸干预氧化损伤的HUVEC作用24和48h后,与ox-FA-def组相比,ox-FA-150、ox-FA-225、ox-FA-300、ox-FA-375 nmol/L组均可以促进细胞增殖(P<0.05);作用72和96 h,与ox-FA-def组相比,叶酸ox-FA-60、ox-FA-150、ox-FA-225、ox-FA-300、ox-FA-375 nmol/L组均可以促进细胞增殖(P<0.05)。结论 150、225、300和375 nmol/L叶酸干预较短时间(24或48 h)或60 nmol/L叶酸干预较长时间(72或96 h)均能降低ox-LDL对HUVEC的氧化损伤,且其作用有明显剂量效应关系。Objective To investigate the protective effects of folic acid on the oxidative damage that ox-LDL （oxi？dized low-density lipoprotein receptor 1） render to human umbilical vein endothelial cells （HUVEC）. Methods HUVECs were injured by ox-LDL （120 mg/L） for 24 h while they were incubated with various concentration of folic acid （0,15, 60, 150, 225, 300, 375 nmol/L）. Then HUVECs were cultured in media contains same concentration of folic acid but without ox-LDL for 72 hours. Finally, HUVECs were harvested after 24, 48, 72 and 96 h. The morphological changes were observed us？ing inverted microscope and cell viability were examined by MTT. Results Various concentrations of folic acid （0,15, 50, 100, 200 and 500 nmol/L） has no obvious promotion or inhibition effect in growth of normal HUVEC （P〉0.05）. However, compared with the ox-FA-def group, 150, 225, 300 and 375 nmol/L of folic acid promoted proliferation of HUVECs with 96 and 120 hours of incubations （P 〈 0.05）. Folic acid of 60, 150, 225, 300 and 375 nmol/L promoted the proliferation of HUVECs with 72 h and 96 hours of incubation （P〈0.05）. Conclusion High dose folic acid can reduce the ox-LDL oxida？tive damage on HUVEC in a concentration dependent manner.

关 键 词：叶酸 剂量效应关系 药物 内皮细胞 脂蛋白类 LDL 

分 类 号：R346.9[医药卫生—基础医学] R349.7