99Tcm-Duramycin用于动脉易损斑块的检测  

Targeting vulnerable plaque of experimental atherosclerosis models with 99Tcm-Duramycin

作　　者：谷珊珊[1] 米宏志[1] 王蒨[1] 苏航[1] 付瑛[1] 牟甜甜[1] 韩洁[1] 

机构地区：[1]首都医科大学附属北京安贞医院核医学科、北京市心肺血管疾病研究所,100029

出　　处：《中华核医学与分子影像杂志》2015年第1期53-58,共6页Chinese Journal of Nuclear Medicine and Molecular Imaging

基　　金：国家自然科学基金（81271613）;北京市自然科学基金（7122060）

摘　　要：目的 采用99Tcm-Duramycin进行动脉粥样硬化（AS）动物模型SPECT/CT显像,探讨该分子探针用于活体AS易损斑块（VP）中凋亡细胞检测的可行性.方法 对16只新西兰大白兔采用完全随机分组方法分成假手术对照组（A组,4只）、稳定斑块组（B组,4只）和VP组（C组,8只）.A组用普通饲料、B、C组用高脂饲料喂养,喂养2周后对动物进行股动脉分离假手术（A、B组）和腹主动脉球囊拉伤术（C组）.分别于喂养第4、第8、第12周对3组按体质量自耳缘静脉注射99Tcm-Duramycin 74 MBq/kg,注射后0.5、1、2h行SPECT/CT显像;并于第4、8周末处死A、B组各1只、C组2只,12周末处死剩余所有动物,进行离体腹主动脉SPECT显像、病理学染色分析和原位末端标记法（TUNEL）细胞凋亡检测,并计算细胞凋亡指数（AI）.采用单因素方差分析处理数据.结果 活体SPECT/CT显像A组始终未见腹主动脉放射性分布,B组在第12周、C组在第8和第12周显像均可见腹主动脉放射性分布.C组第12周腹主动脉局部放射性异常浓聚,T/NT为3.40±0.22,明显高于A、B2组的T/NT （2.12±0.65、2.68±0.18,F=198.775,P＜0.05）,与腹主动脉离体显像及病理染色结果一致.3组动物12周末腹主动脉片段TUNEL测得的细胞AI分别为（0±0.02）％、（5.3±1.97）％和（25.4±6.32）％,C组的AI明显高于A、B2组（F=70.260,P＜0.05）.结论 99Tcm-Duramycin可无创性检测AS的VP中的凋亡细胞,可为评价AS的稳定性提供信息.Objective To investigate the value of 99Tcm-Duramycin SPCET/CT in the detection of vulnerable plaque （VP） in atherosclerotic （AS） rabbit models.Methods Sixteen New Zealand male rabbits were randomly divided into three groups：group A （sham-operated group,n =4）,group B （stable plaque group,n =4） and group C （VP group,n =8）.Group A was fed on normal diet,and the other 2groups were fed on cholesterol diet for 12 weeks.Femoral artery dissection sham-operation was done in group A and group B,while balloon-induced abdominal aorta wall injury was produced in group C after 2 weeks39;feeding.Animals were injected with 99Tcm-Duramycin （74 MBq/kg） and then SPECT/CT imaging was performed at the end of 4,8,and 12 weeks,respectively.Abdominal aortas were explanted for ex vivo imaging and histological characterization of plaque.The apoptosis index （AI） was calculated.One-way analysis of variance was used to analyze data.Results There was no radioactive uptake by the abdominal aorta in each group at the end of 4 weeks and no uptake in group A and group B at the end of 8 weeks.There was slightly uptake radioactive uptake by the abdominal aorta in group B at the end of 12 weeks and in group C at the end of 8 weeks.There was intense uptake at the lesions of AS rabbits in group C at the end of 12 weeks,and the T/NT value significantly higher than that of the other two groups （3.40±0.22 vs 2.12±0.65,2.68±0.18,F=198.775,P〈0.05）.The result was confirmed in the ex vivo imaging of the explanted aorta.The AI of group C was significantly higher than that of group A and B （（25.4±6.32） % vs （0±0.02）%,（5.3± 1.97）%,F=70.260,P〈0.05）.Conclusions 99Tcm-Duramycin scimigraphy could identify the apoptosis of VP in the rabbit AS models.It is a promising non-invasive method to diagnose AS plaques.

关 键 词：动脉粥样硬化 细胞凋亡 锝 体层摄影术 发射型计算机 单光子 体层摄影术 X线计算机 兔 

分 类 号：R543.5[医药卫生—心血管疾病]