荧光素钠在切伦科夫能量转移中的应用  

Application of fluorescein sodium on Cerenkov radiation energy transfer

作　　者：何耘[1,2] 屈亚威[1] 宋天明[3] 张晓军[4] 白净[2] 刘海峰[1] 

机构地区：[1]武警总医院消化内科,北京100039 [2]清华大学生物医学工程系 [3]中国科学院自动化研究所 [4]解放军总医院核医学科

出　　处：《中华核医学与分子影像杂志》2015年第1期59-62,共4页Chinese Journal of Nuclear Medicine and Molecular Imaging

基　　金：吴阶平医学基金（320.6750.12121）

摘　　要：目的 分析医用造影剂荧光素钠作为18 F-FDG的切伦科夫光子能量转移（CRET）媒介的应用价值和适宜应用方案.方法 将不同浓度（0.05、0.10、0.20、1.00、2.00、4.00和8.00 mmol/L）的荧光素钠溶液混合1.85 MBq 18F-FDG进行CLI,观察CRET效应.勾画ROI,定量分析光学信号强度,研究荧光素钠浓度对CRET效应的影响,并确定产生最佳信号增强效果的浓度.将此浓度和对照浓度的荧光素钠溶液混合1.85～ 11.10 MBq 18F-FDG进行CLI,勾画ROI并进行直线拟合分析,研究该最佳浓度的适用范围和核素剂量对CRET效应的影响.将荷瘤裸鼠注射18F-FDG后进行CLI,之后注射荧光素钠溶液再次成像,对比前后图像信号强度,观察CRET效应对活体CLI的改善效果.结果 体外实验表明,荧光素钠混合18F-FDG能有效产生CRET效应.1.00 mmol/L荧光素钠溶液在1.85～ 11.10 MBq 18F-FDG范围内能达到最佳光学信号增强效果,为单独18F-FDG的3.7倍.肿瘤模型实验表明荧光素钠和18F-FDG在生物组织内亦能有效产生CRET,增强信号强度.结论 荧光素钠混合18 F-FDG所产生的CRET效应能提高切伦科夫辐射的光学信号强度,有效缩短曝光时间,获得更高的信噪比和对比度.Objective To explore and evaluate the method and feasibility of applying the contrast agent fluorescein sodium to enhance the efficiency of Cerenkov radiation energy transfer （CRET）.Methods Fluorescein sodium was employed as the energy transfer receptor of 18 F-FDG Cerenkov radiation （CR） to achieve stronger signal.18F-FDG was mixed with fluorescein sodium of different concentrations：0.05,0.10,0.20,1.00,2.00,4.00,8.00 mmol/L.Optical signal strength was quantified by ROI analysis.The influence of fluorescein sodium concentration on CRET efficiency and the optimal concentration that could achieve most optical signal augmentation were studied.The optimal concentration of fluorescein sodium was mixed with 1.85-11.10 MBq 18F-FDG for CLI.Linear regression was performed to investigate the range of 18F-FDG dose with most optical signal augmentation and the influence of 18F-FDG dose on CRET efficiency.A tumorbearing nude mouse was imaged after intravenous injection of 18F-FDG and then imaged again after injection of fluorescein sodium post 18 F-FDG injection.These two sets of images were compared to evaluate the CRET efficiency in vivo.Results In vitro study showed that 1.00 mmol/L fluorescein sodium was the optimal concentration for 18F-FDG dose ranging from 1.85 MBq to 11.10 MBq.The signal from 18F-FDG mixed with fluorescein sodium was 3.7 times stronger than that from 18F-FDG alone.Experiment in vivo suggested that 18F-FDG mixed with fluorescein sodium could effectively enhance the signal strength by CRET.Conclusion 18F-FDG mixed with fluorescein sodium may improve the efficiency of CRET,therefore,can enhance the signal strength,reduce the exposure time and achieve better signal to noise ratio.

关 键 词：切伦科夫光学成像 能量转移 荧光素 

分 类 号：R817[医药卫生—影像医学与核医学]