白细胞精子症患者精液质量参数变化的初步研究  

Semen quality and quantity parameters of patients with leukocytospermia

作　　者：何磊[1] 万小妍[1] 徐杰伟[1] 梁晓丽[1] 廖黎黎[1] 黄永汉[1] 

机构地区：[1]佛山市第一人民医院妇产科生殖医学中心,广东佛山528000

出　　处：《热带医学杂志》2015年第3期328-330,361,共4页Journal of Tropical Medicine

摘　　要：目的研究白细胞精子症患者精液质量参数的变化。方法根据WHO推荐方法对120例男性患者的精液质量进行分析,采用白细胞过氧化物酶法检测精液白细胞浓度,并将患者分为白细胞精子症组(白细胞浓度≥1×106/ml)和对照组(白细胞浓度<1×106/ml)。结果与对照组比较,白细胞精子症组精液体积(3.5 ml vs 3.8 ml)、精子浓度(112.4×106/ml vs 113.7×106/ml)和前向运动精子百分率(48.8%vs 46.7%)差异均无统计学意义(P>0.05);精子正常形态率(7.1%vs 10.4%,P<0.05)和精子存活率(73.8%vs 83.3%,P<0.05)较低,精子DNA碎片指数较高(15.9%vs 7.4%,P<0.05);而且,白细胞精子症患者的精子正常形态率和精子存活率均与白细胞浓度呈负相关(R=-0.56,P<0.01;R=-0.622,P<0.01);精子DNA碎片指数与白细胞浓度呈正相关(R=0.886,P<0.01)。结论精液中白细胞浓度对精液体积、精子浓度、前向运动精子百分率无明显影响;精液中白细胞浓度高于正常可导致精子正常形态率和存活率降低,精子DNA碎片指数增高。Objective To explore the changes of semen quality and quantity parameters in patients with leukocytospermia.Methods Semen quality and quantity of 120 male outpatients were assessed according to WHO standard, and leukocytes concentration in semen was determined by peroxidase method. Based on these measurements thesamples were divided into two groups, leukocytospermia group and control group. Results Compared to the control, there was no difference about semen volume（3.5 ml vs 3.8 ml,P〉0.05）, sperm density（112.4×10^6/ ml vs 113.7×10^6/ ml,P〉0.05）,and forward progression percentage（48.8% vs 46.7%,P〉0.05）, while sperm nomal morphology（7.1% vs 10.4%,P〈0.05） and survival rate（73.8% vs 83.3%,P〈 0.05） were lower, and sperm DNA fragment index（DFI） was higher（15.9% vs 7.4%,P〈0.05） in leukocytospermia group. The percentage of sperm normal morphology（R =-0.56,P 〈0.01）and sperm survival rate（R=-0.622,P〈0.01） were all negatively correlated to leukocyte concentration; DFI was positively correlated to leukocyte concentration（R=0.886,P〈0.01）. Conclusion Leukocyte concentration has no effect on semen volume, sperm density and forward progression percentage; paranormal leukocyte in semen can result in a decrease in sperm normal morphology percentage and survival rate, while an increase in sperm DFI.

关 键 词：白细胞精子症 精液参数 精子DNA碎片指数 

分 类 号：R698.2[医药卫生—泌尿科学]