辛伐他汀对体外培养鼠骨形成蛋白BMP-2基因的调控  

Regulation of Simvastatin on Bone Morphogenetic Protein-2 Gene of Rat in Vitro

作　　者：芮丹云[1] 唐丽媛[1] 陈洁[2] 鲁宁 

机构地区：[1]昆明学院医学院,云南昆明650214 [2]云南省第一人民医院,云南昆明650032 [3]昆明医科大第二附属医院骨科,云南昆明650101

出　　处：《昆明医科大学学报》2015年第4期23-26,共4页Journal of Kunming Medical University

摘　　要：目的研究辛伐他汀对鼠骨形成蛋白(bone morphogenetic protein-2,BMP-2)及其m RNA表达的影响,验证辛伐他汀是否能够促进鼠成骨细胞的骨形成.方法将兔子分为假手术组,模型组和辛伐他汀组3个小组,每组6只.在辛伐他汀组用药1月、2月和4月时,收集各组兔子的耳中央动脉血清,将上述血清加入鼠MC3T3-E1细胞培养4d后提取总RNA,逆转录出骨形成蛋白BMP-2的cDNA后进行实时荧光定量PCR,对产物进行检测.结果用药1个月,辛伐他汀组BMP-2表达量较假手术组高2.79倍(P=0.0004),较模型组高2.66(P=0.001);用药2个月,辛伐他汀组BMP-2表达量较假手术组高1.88倍,较模型组高1.75倍(P=0.001);用药4个月,辛伐他汀组BMP-2表达量较假手术组高0.86倍(P=1.000),较模型组高1.04倍.结论辛伐他汀对骨形成有正效应.Objective To study the effect of simvastatin on the expression of proteins and mRNA in bone morphogenetie protein-2 （BMP-2） gene of rat osteoblasts, then confirm whether simvastatin can promote the bone formation in rats. Methods Rabbits were divided into three groups： sham-operated group, osteoporosis model group and simvastatin group, with six rabbits in each group. Rabbits ear arteries blood serums were collected respectively after drug intervention one, two and four months, which were used to culture MC3T3-E1 cell. Total RNA was extracted after four days of culture, then cDNA of BMP-2 was obtained using reverse transcription and all products were detected by real time quantitative PCR （RQ-PCR） technique. Results After one, two and four months, the BMP-2 expression quantity of simvastatin group was 2.79 （P=0.004）, 1.88 （P=0.005） and 0.86 （P=0.926） times higher than that of sham-operated group, and 2.66, 1.75 （P=0.009） and 1.04 （P= 1.000） times higher than that of osteoporosis model group, respectively. Conclusion Simvastatin has a positive effect on bone formation.

关 键 词：辛伐他汀 骨形成蛋白-2 荧光定量PCR 

分 类 号：R523[医药卫生—内科学]