人参皂苷Rg1保护UVB所致皮肤成纤维细胞急性损伤  

Protective effects of ginsenoside Rgl against UVB-induced photo-damage on skin fibroblasts

作　　者：刘勇[1] 徐浩翔[2,3] 惠云[4] 张晓峰[2,3] 肖学敏[2,3] 王宝玺[2,3] 

机构地区：[1]新疆维吾尔自治区人民医院皮肤科,乌鲁木齐830002 [2]中国医学科学院北京协和医学院皮肤病研究所,南京210042 [3]江苏省皮肤与性病分子生物学重点实验室,南京210042 [4]南京军区南京总医院皮肤科,南京210042

出　　处：《中国麻风皮肤病杂志》2015年第3期136-139,共4页China Journal of Leprosy and Skin Diseases

基　　金：国家自然科学基金资助项目(编号:81101207;81472905);教育部高校博士点基金(编号:20111106120052);中央高校基本科研业务费专项资金&北京协和医学院青年基金(编号:3332013056);杨森科研基金(编号:JRCC2011-皮-01)

摘　　要：目的:确定人参皂苷Rg1对UVB损伤成纤维细胞的保护作用。方法:体外培养人皮肤成纤维细胞,分为正常对照组、UVB照射组、UVB照射+人参皂苷Rg1组(5,10和20μg/mL),MTT法检测细胞增殖活性,ELISA法检测细胞中SOD活性、MDA和细胞上清液中MMP-1和MMP-3的含量,Western blot法检查细胞中MMP-1和MMP-3的表达。结果:UVB联合不同浓度人参皂苷Rg1组较UVB照射组,细胞SOD活性升高,MDA、MMP-1和-3含量均有不同程度下降,人参皂苷Rg1浓度越高作用越明显。结论:人参皂苷Rg1对UVB造成的皮肤成纤维细胞损伤具有保护作用。Objective：To determine the protective effects of ginsenoside Rg1 against UVB-induced photo-damage on skin fibroblasts. Methods：Human skin fibroblasts cultured in vitro were divided into normal control group, UVB group, UVB combined ginsenoside Rg1 groups （concentration of 5, 10 and 20 μ g/mL）. The proliferation activity of cells was detected by MTT assay. SOD activity, MDA and the content of MMP-1 and MMP-3 were detected by ELISA. The expression of MMP-1 and MMP-3 proteins was detected through Western blot. Results：The SOD activity in UVB combined ginsenoside Rg1 groups was higher than that in UVB group. The content of MMP-1 and MMP-3 in UVB combined ginsenoside Rg1 groups was lower than that in UVB group. The effect was dose dependent. Conclusion： Ginsenoside Rg1 can protect the dermal fi-broblasts from UVB damage.

关 键 词：人参皂苷RG1 皮肤成纤维细胞 UVB 

分 类 号：R285[医药卫生—中药学]