尖锐湿疣皮损中p16基因启动子区高甲基化及其mRNA的表达  

The hypermethylation of p16 gene promoter region and the expression of p16 mRNA in condyloma accuminatum

作　　者：许辉[1] 马红[1] 闵敏[1] 顾文涛[1] 李遇梅[1] 

机构地区：[1]江苏大学附属医院皮肤科,江苏镇江212000

出　　处：《中国麻风皮肤病杂志》2015年第3期180-182,共3页China Journal of Leprosy and Skin Diseases

基　　金：镇江市科技局基金资助项目(编号:SH2008040)

摘　　要：目的:明确尖锐湿疣组织中p16基因启动子区高甲基化与p16 mRNA表达的关系。方法:采用甲基化特异性PCR技术检测30例尖锐湿疣组织及20名正常对照组织p16基因启动子区CpG岛甲基化状态,用RT-PCR法检测p16 mRNA的表达。结果:尖锐湿疣组织p16基因启动子高甲基化率为26.67%(8/30),正常对照组高甲基化率为0,差异具有统计学意义(P<0.05)。高甲基化的尖锐湿疣组织p16 mRNA的表达高于未甲基化的尖锐湿疣组织p16 mRNA的表达和高于正常对照组织p16mRNA的表达。结论:尖锐湿疣组织皮损中p16基因启动子区甲基化不导致p16 mRNA表达的缺失。Objective：To determine the relationship between the hypermethylation of the p16 gene promoter region and the expression of p16 mRNA in condyloma accuminatum （ CA） tissue. Methods： The hyperm-ethylation of p16 gene promoter CpG island and the expression of p16 mRNA were detected by methylation-specific PCR and RT-PCR respectively in CA tissue from 30 patients and 20 healthy controls. Results： The rates of the hypermethylation of p16 gene promoter in CA tissue and normal skin were 26.67% （8/30） and 0, with a significant difference （ P〈0.05） . The expression of p16 mRNA in hypermethylation CA tissue was higher than that in unmethylated CA tissue and normal tissue. Conclusion：The hypermethylation of p16 gene promoter region does not lead to the loss of p16 mRNA expression in CA tissue.

关 键 词：尖锐湿疣 人乳头瘤病毒 甲基化 P16 

分 类 号：R752.53[医药卫生—皮肤病学与性病学]