2010年至2012年深圳地区献血者隐匿性乙型肝炎病毒感染的分子病毒学特征  

Molecular virological characteristics of occult hepatitis B virus infection in blood donors in Shenzhen from 2010 to 2012

作　　者：周姗[1] 杜鹏[2] 郑欣[2] 叶贤林[2] 曾劲峰[2] 卢亮[2] 朱为刚[2] 杨宝成[2] 李婷婷[1] 黎诚耀[1] 

机构地区：[1]南方医科大学生物技术学院输血医学系,广州510515 [2]深圳市血液中心

出　　处：《中华传染病杂志》2015年第3期150-153,共4页Chinese Journal of Infectious Diseases

基　　金：国家自然科学基金资助项目（81071348;81371801）

摘　　要：目的探讨深圳地区隐匿性HBV感染相关的HBV分子病毒学特征。方法从2010年4月至2012年12月深圳市血液中心采集的310167份无偿献血者标本中筛查HBsAg阴性HBV DNA阳性标本，荧光定量PCR检测HBV DNA水平，套式PCR扩增HBVS区基因，分析隐匿性HBV感染相关HBV的分子生物学特征。使用MEGA软件以邻接法构建系统进化树进行基因分型。结果筛查出121份HBsAg阴性HBV DNA阳性标本，99份确认为隐匿性HBV感染，其中69份可分型的样品中B基因型57份，C基因型12份。在主要亲水区（MHR）氨基酸的变异中，B基因型隐匿性HBV感染毒株突变频率最高的是F134I／L，出现12次；C基因型MHR区域未出现明显的氨基酸变异。其他S区蛋白序列中，B基因型10株出现P105R、10株出现Q101R／H／K、9株出现G102I、V177A、9株出现S39N等氨基酸置换；C基因型10株出现$53L氨基酸置换。结论S蛋白氨基酸的置换、插入、终止可能影响HBV的复制与蛋白的表达，与隐匿性HBV感染的发生和发展密切相关。Objective To investigate the molecular virological characteristics of occult hepatitis B virus （HBV） infection （OBI） in Shenzhen. Methods A total of 310 167 blood samples were collected from donors between April 2010 and December 2012 in Shenzhen Blood Center. Hepatitis B surface antigen （HBsAg） /HBV DNA＋ samples were screened. HBV DNA was quantified by quantitative polymerase chain reaction （PCR）. S region was tested by nested PCR. Molecular virological characteristics of OBI samples were further analyzed. Genotyping was conducted by MEGA software to construct the phylogenetic tree by neighbor-joint. Results Among 121 HBsAg^-/HBV DNA^＋ samples, 99 samples were identified as OBI. Genotyping was conducted in 69 samples, with 57 of genotype B and 12 of genotype C. F134I/L mutation in 12 samples in the major hydrophilic region （MHR） of amino acids was the most frequent mutation in B genotype strains, and there was no obvious amino acid mutation in C genotype strains. In other S fragment sequences, 10 of the B genotype OBI stains had P105R, 10 had Q101R/H/K, 9 had G102I, V177A and 9 had S39N amino acid replacements. While 10 of the C genotype OBI strains had S53L amino acid substitution. Conclusions Mutation, insertion and termination of amino acids in S protein may influence HBV replication and protein expression, which is related with the emergence and development of OBI.

关 键 词：肝炎病毒 乙型 隐匿性感染 供血者 基因型 分子病毒学特征 

分 类 号：R512.62[医药卫生—内科学]