IL-31对肺泡Ⅱ型上皮细胞A549表达CCL2的影响  

Effect of IL-31 on the expression of CCL2 in type Ⅱ alveolar epithelial cells A549

作　　者：高婧[1] 杜青波[1] 谭艳芳[1] 岳欢[1] 黄俊琼[1,2] 

机构地区：[1]遵义医学院检验系,贵州遵义563099 [2]遵义医学院附属医院检验科,贵州遵义563099

出　　处：《现代医药卫生》2015年第8期1121-1123,共3页Journal of Modern Medicine & Health

基　　金：国家自然科学基金资助项目(81260266);贵州省优秀科技教育人才省长专项基金项目(黔省专合字[2009]51号)

摘　　要：目的通过体外实验研究IL-31对肺泡Ⅱ型上皮细胞A549表达趋化因子C-C趋化因子配体2(CCL2)的影响,探讨IL-31在支气管哮喘气道炎症中的作用。方法体外培养肺泡Ⅱ型上皮细胞A549,用不同浓度(10、50、100 ng/m L)IL-31作用A549细胞、相同浓度(10 ng/m L)IL-31作用A549细胞不同时间(12、24、36 h),收集细胞,提取细胞总RNA,荧光定量PCR法分析趋化因子CCL2 m RNA的表达情况。结果不同浓度IL-31作用于A549细胞后,趋化因子CCL2的表达较正常未处理组明显增加,差异有统计学意义(P<0.05);10 ng/m L IL-31作用A549细胞后,不同时间段CCL2的表达均增高,且差异有统计学意义(P<0.05)。结论 IL-31作用于肺泡Ⅱ型上皮细胞A549后,趋化因子CCL2的表达明显增高,表明IL-31可能诱导肺组织细胞分泌趋化因子参与哮喘气道炎症过程。Objective To explore the effect of of IL-31 on airway inflammation of bronchial asthma by studying the effect of IL-31 on the expression of CCL2 in type Ⅱ alveolar epithelial cells A549 in vitro. Methods The type Ⅱ alveolar epithelial cells A549 were cultured in vitro,with the different-concentration IL-31 solution including 10,50,100 ng/m L acting on them and the same concentration IL-31（10 ng/m L）acting on the them for different times including 12,24 h and 36 h,and then collected the cells and extracted the total cellular RNA. The m RNA expression of chemokine CCL2 were measured by fluorescent real-time PCR assay. Results Compared with the normal treatment group,the m RNA expression of chemokine CCL2 was significantly increased with different concentrations of IL-31 impacting on the A549 cells. The difference was statistically significant（P〈0.05）;The m RNA expression of chemokine CCL2 was increased with 10 ng/m L IL-31 impacting on the A549 cells at different time periods. The difference had statistical significance（P〈0.05）. Conclusion The expression of chemokine CCL2 is obviously increased with the impact of IL-31 acting in type II alveolar epithelial cells A549,which indicates that IL-31 may probably induce lung tissue cells to secrete chemokines involving in asthmatic airway inflammation.

关 键 词：趋化因子CCL2 白细胞介素类 肺泡/细胞学 哮喘 气管炎 

分 类 号：R563[医药卫生—呼吸系统]