环介导等温扩增技术及其在动物疫病检测中的应用  

Loop-mediated Isothermal Amplifi cation and Its Application in Animal Disease Diagnosis

作　　者：胡成[1] 成大荣[1] 余树培[1] 赵方[1] 苏玲玲[1] 蔡树东[1] 顾玲玲[1] 王永娟[2] 

机构地区：[1]扬州大学兽医学院,江苏扬州225009 [2]江苏农牧科技职业学院,江苏泰州225300

出　　处：《中国动物检疫》2015年第4期46-50,共5页China Animal Health Inspection

基　　金：江苏省博士后科研资助计划项目(1401077B);国家自然科学基金项目(31302096);江苏农牧科技职业学院2014年度重点支持项目(NSFZD1405);扬州朝天歌农牧科技有限公司横向合作项目(00010114012)

摘　　要：环介导等温扩增(LAMP)是一门新兴的分子生物学检测技术,因其具有高特异性、高灵敏度、简便快捷、成本低廉等特点,受到越来越多兽医工作者的关注。目前,该方法已经广泛应用于各种病原微生物的检测。本文就环介导等温扩增技术的原理、方法、应用等方面进行简述,最后指出LAMP当前存在的一些不足之处,并对其发展前景进行了展望,以期为其临床应用提供理论依据。The loop-mediated isothermal amplification（LAMP）is a new nucleic acid amplification method. Due to its high specificity,efficiency,simplicity,rapidity and cheapness,more and more veterinarians focus on the method. At present,LAMP has been widely used in detecting various pathogens. In this review,the principle,process and application of LAMP were introduced,its short-comings were pointed out and the future development in animal disease diagnosis was prospected in order to provide theoretical basis for clinical application.

关 键 词：环介导等温技术 动物疫病 应用 检测 

分 类 号：S854.4[农业科学—临床兽医学]