星点设计-效应面法优化常春藤皂苷C提取工艺  

Optimization of Extraction Process of Hederacoside C by Central Composite Design and Response Surface Method

作　　者：曾建刚[1] 孙化鹏[1] 阮琴妹 程建宇[1] 钟晓红[1] 

机构地区：[1]湖南农业大学园艺园林学院,湖南长沙410128

出　　处：《湖南农业科学》2015年第2期91-94,98,共5页Hunan Agricultural Sciences

基　　金：湖南省研究生科研创新项目(CX2014B290)

摘　　要：以常春藤皂苷C为指标,采用星点设计考察乙醇浓度、回流温度以及提取时间对提取工艺的影响,对结果进行多元线性回归和二项式拟合,用效应面法优化得出回流提取的最佳工艺条件,以期探讨回流提取常春藤叶片中常春藤皂苷C的最佳工艺。结果表明,二项式拟合方程各项指标优于多元线性方程,优化后的常春藤皂苷C的最佳提取工艺条件为乙醇浓度62.6%,回流温度82.3℃,提取时间54.7 min,回流提取2次,预测得率与实测得率偏差为1.12%。星点设计-效应面法优化常春藤皂苷C的提取工艺方法可行,预测性良好。In order to explore the optimized extractive techniques of Hederacoside C from the leaves of Hedera. helix by reflux extraction,the effects of influence factors such as ethanol concentration, extract temperature and extract time on extraction rate of Hederacoside C were investigated by using box-behnken design and response surface method. The data were imitated by using multi-linear equation and second-order polynomial equation. The results showed that multiple indicators from second-order polynomial equation were prior to those from multi-linear equation. Analysis indicated the optimum conditions were 62.6%, ethanol reflux extract at 82.3℃ for 2 times, and each time for 54.7min. Bias between predicted and measured values was 1.12%. we can draw the conclusion that the optimum model was highly predictive and feasible.

关 键 词：常春藤皂苷C 效应面法 星点设计 回流提取工艺 

分 类 号：R285.5[医药卫生—中药学]