海滩牵牛正丁醇提取物化学成分的抗炎活性研究  

Anti-inflammatory activity of chemical components from n-butanol extract of Ipomoea stolonifera

作　　者：郑付春[1] 陈一村[2] 秦永洁[2] 蔡聪艺[2,3] 

机构地区：[1]汕头大学医学院第一附属医院药剂科,广东汕头515041 [2]汕头大学医学院药理学教研室,广东汕头515041 [3]汕头大学医学院第二附属医院药剂科,广东汕头515041

出　　处：《癌变．畸变．突变》2015年第2期146-149,153,共5页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：广东省自然科学基金-团队项目(9351503102000001);中央财政支持地方高校发展专项资金

摘　　要：目的:对潮汕地区草药海滩牵牛(IS)正丁醇提取物(BE-IS)化学成分进行分离纯化,并研究这些成分在体内的抗炎活性。方法:利用多种色谱法对BE-IS中的化学成分进行分离,通过波谱解析方法确定它们的结构;在巴豆油致小鼠耳肿胀实验、角叉菜胶致大鼠足肿胀实验等炎症模型上评价BE-IS化学成分的抗炎活性。结果:BE-IS分离得到5个单体,分别为东莨菪亭、伞形花内酯、秦皮乙素、橙皮素和姜黄素。与对照组比较,BE-IS单体均能不同程度地抑制巴豆油所致小鼠耳肿胀、角叉菜胶所致大鼠足肿胀,差异均有统计学意义(P<0.01或P<0.05)。结论:首次从海滩牵牛分离得到的5个单体化合物具有较好的体内抗炎活性,揭示了海滩牵牛抗炎活性的物质基础。OBJECTIVE： To separate and refine the chemical components from n-butanol extract of Ipomoea stolonifera （BE-IS） in Chaoshan area, and investigate their anti-inflammatory activities in vivo. METHODS： The chemical components from BE-IS were separated by different kinds of chromatography and identified by spectrum analysis methods. Then anti-inflammatory activity was evaluated in croton oil-induced ear edema model and carrageenan-induced paw edema model. RESULTS： Scopoletin, umbelliferone, esculetin, hesperetin and curcumin were extracted from BE-IS. Compared to the control group, compounds from BE-IS could inhibit the edema induced by croton oil or carrageenan to different degree, and the difference was statistically significant （P〈0.01 or P〈0.05）. CONCLUSION： The chemical components from BE-IS have anti-inflammatoty activities, which explained why Ipomoea stolonifera possess strong anti-inflammatoty activity.

关 键 词：海滩牵牛 化学成分 抗炎活性 香豆素 姜黄素 

分 类 号：R285[医药卫生—中药学]