大鼠骨髓间充质干细胞对细菌性前列腺炎抑制作用的研究  

Bone marrow mesenchymal stem cells suppress E coli-induced bacterial prostatitis in rats

作　　者：韩广玮[1,2] 刘城城[1] 高文宏[3] 崔栋[1] 易善红[1] 

机构地区：[1]第三军医大学附属新桥医院泌尿外科,重庆400037 [2]解放军169医院泌尿外科,湖南衡阳421002 [3]第三军医大学附属新桥医院超声诊断科,重庆400037

出　　处：《中华男科学杂志》2015年第4期294-299,共6页National Journal of Andrology

基　　金：国家自然科学基金项目(81470926);第三军医大学临床科研基金资助项目(2009XLC22)~~

摘　　要：目的：研究外源性骨髓间充质干细胞（BMSCs）对大肠埃希菌致大鼠前列腺炎的抑制作用。方法：贴壁选择法分离、培养、扩增BMSCs。将sD大鼠50只随机分为急性细菌性前列腺炎（ABP）组、ABP＋BMSCs组、慢性细菌性前列腺炎（CBP）组、CBP＋BMSCs组和正常对照组，每组10只。在小动物超声引导下向大鼠前列腺两侧叶内注射大肠埃希菌建立前列腺炎症模型，注入细菌1～14d为急性炎症期，4—12周为慢性炎症期，对照组注入等量PBS。然后将BMSCs注入ABP＋BMSCs组和CBP＋BMSCs组大鼠前列腺内，观察移植BMSCs2周后大鼠前列腺病理学变化并做炎症评分，RT—PCR扩增前列腺组织中炎症因子IL—β、TNF—αmRNA，ELISA检测IL-1β、TNF-α蛋白含量，统计分析各组表达差异。结果：前列腺组织病理学提示炎症组前列腺组织呈典型炎症病理变化，腺管结构改变，间质水肿，炎细胞浸润，纤维组织增生，而BMSCs治疗组大鼠前列腺炎症明显减轻。PCR和ELISA分析显示ABP组IL-1βmRNA（0．829±0．121）、蛋白（271．75±90．59）pg／ml和TNF—αmRNA（0．913±0．094）、蛋白（105．78±19．05）pg／ml含量均显著高于对照组[（0．3424±0．087）、（45．76±17．99）pg／m1]、[（0．247±0．054）、（19．42±7．75）pg,／ml]（P均〈0．01）及BMSCs治疗组[（0．433±0．072）、（51．34±22．13）pg／m1]、[（0．313±0．076）、（28．38±8．78）pg／m1]（P〈0．01）；CBP组IL-1βmRNA（0．975±0．114）、蛋白（265．31±71．34）pg／ml和TNF-理mRNA（0．886±0．084）、蛋白（107．45±26．11）pg／ml含量也均显著高于对照组及BMSCs治疗组[（0．396±0．064）、（56．37±21．22）pg／m1]、[（0．417±0．068）、（29．21±10．22）pg／m1]（P均〈0．01）。结论：注入BMSCs能够减轻大肠埃希菌引起的前列腺炎症反应，其作用可能与减少炎细胞浸润，降低IL-1β、TNF-α水平有Objective： To investigate the inhibitory effect of bone marrow mesenchymal stem cells （BMSCs） on E coil-induced prostatitis in rats. Methods ： BMSCs were isolated, cultured and amplified by the attached choice method. Fifty SD rats were random- ized into five groups of equal number： normal control, acute bacterial prostatitis （ABP）, chronic bacterial prostatitis （CBP）, ABP ＋ BMSCs, and CBP ＋ BMSCs, and the animals in the latter four groups were injected with E. coli into both sides of the prostate under ul- trasound guidance for 1 - 14 days to induce ABP and for 4 - 12 weeks to induce CBP. The control rats were injected with the same amount of PBS. Two weeks after injection of BMSCs into the prostates, patbomorphological changes in the prostate were observed under the light microscope and the mRNA and protein levels of IL-113 and TNF-ct determined by RT-PCR and ELISA, respectively, followed by statistical analysis with SPSS 18.0. Results ： Histopathological evaluation showed typical pathological inflammatory changes in the prostates of the rats in the ABP and CBP groups, including glandular structural changes, interstitial edema, inflammatory cell infiltra- tion, and fibrous hyperplasia, which were all remarkably relieved after treated with BMSCs. The mRNA and protein levels of IL-1 β（ [ 0.829 ± 0. 121 ] and [ 271.75 ± 90.59 ] pg/ml） and TNF-α （ [ 0.913 ± 0. 094 ] and [ 105.78 ± 19.05 ] pg/ml） in the ABP and those of IL-1 [3 （ [ 0. 975 ± 0. 114 ] and [ 265.31 ± 71.34 ] pg/ml） and TNF-c~ （ [ 0. 886 ＋ 0. 084 ] and [ 107.45 ＋ 26.11 ] pg/ml） in the CBP groups were significantly higher than those in the control rats （ [ 0. 342 ± 0. 087 ] and [ 45.76 ± 17.99 ] pg/ml, P 〈 0.05 ） ; （ [ 0. 247 ± 0. 054 ] and （ [ 19.42 ± 7.75 ] pg/ml, P 〈 O. 01 ） as well as than those in the ABP ＋ BMSCs （ [ 0. 433 ± 0. 072 ] and [51.34±22.13] pg/ml, P〈0.05）; （[0.313±0.076] and [28.38 ~8.78] pg/ml, P〈0.01） and the CBP＋BMSCs group （[0.396±0.064] and [

关 键 词：髓间充质干细胞 细菌性前列腺炎 炎症因子 TNF—α IL-1Β 大鼠 

分 类 号：R697.33[医药卫生—泌尿科学]