灭活轮状病毒疫苗与灭活脊髓灰质炎病毒疫苗联合使用免疫效果评价  

Immune effects of inactivated rotavirus vaccine combined with inactivated poliovirus vaccine

作　　者：牛香莲 张光明[1] 吴晋元[1] 米鍇 谢力[1] 孙晓琴[1] 李鸿钧[1] 孙茂盛[1] 

机构地区：[1]中国医学科学院北京协和医学院医学生物学研究所分子生物学研究室云南省重大传染病疫苗研发重点实验室,云南昆明650118

出　　处：《中国生物制品学杂志》2015年第3期217-222,共6页Chinese Journal of Biologicals

基　　金：十二五重大新药创制(2014ZX09102041004);十二五"863"项目(2012AA02A404);云南省应用基础面上项目(2013FZ140)

摘　　要：目的评价灭活轮状病毒疫苗(inactivated rotavirus vaccine,IRV)与灭活脊髓灰质炎病毒疫苗(inactivated poliovirus vaccine,IPV)免疫大鼠后,两种疫苗间免疫效果的相互作用。方法将Wistar大鼠随机分成联合免疫组、间隔免疫组和单疫苗免疫组:联合免疫组接种IPV后4周,经双侧腿同时免疫IPV和IRV,共2次,间隔4周;单疫苗免疫组2组,分别免疫3针IPV和2针IRV,每针均间隔4周;间隔免疫组免疫1针IPV后4周,开始进行IPV和IRV间隔免疫(每针间隔2周,共6周)。分别于每次免疫后4周经尾静脉采血,分离血清,采用微量中和试验结合免疫荧光抑制方法检测血清抗RV中和抗体水平,ELISA法检测血清抗RV特异性Ig G抗体水平,微量中和试验法检测血清抗脊髓灰质炎病毒中和抗体水平。结果大鼠经2针IRV免疫后,血清抗RV中和抗体和特异性Ig G抗体几何平均滴度(geometric mean titer,GMT)均明显上升,血清抗体阳转率均达100%;经3针IPV免疫后,Ⅰ、Ⅱ、Ⅲ型脊髓灰质炎病毒中和抗体GMT均明显上升,抗体阳转率均达100%;联合免疫组、间隔免疫组和单疫苗免疫组间血清抗RV中和抗体水平、特异性Ig G抗体水平及抗脊髓灰质炎病毒中和抗体水平差异无统计学意义(P>0.05)。结论 IRV和IPV免疫大鼠后均获得到良好的免疫效果,两种疫苗联合使用时,免疫效果无相互干扰作用。Objective To evaluate the immunogenicity and interaction of co-vaccination with inactivated rotavirus vaccine（IRV）and inactivated poliovirus vaccine（IPV）in rats. Methods Wistar rats were randomly divided into co-vaccination,alternative vaccination as well as IPV and IRV groups. The rats in co-vaccination group were vaccinated with IPV at first and, 4 weeks later, vaccinated twice with IPV and IRV in right and left posterior limbs respectively, at an interval of 4weeks. The rats in IPV and IRV groups were vaccinated with three doses of IPV and two doses of IRV respectively, each at an interval of 4 weeks. However, the rats in alternative vaccination group were vaccinated with IPV at first and, 4 weeks later, alternatively vaccinated with IPV and IRV for 6 weeks, each at an interval of 2 weeks. Venous blood samples were collected 4 weeks after each vaccination, from which sera were separated and determined for neutralizing antibody levels against rotavirus（RV）and poliovirus by microneutralization test, and for specific Ig G level against RV by ELISA. Results Both geometric mean titers（GMTs）of neutralizing antibody and specific Ig G against RV of rats after vaccination with two doses of IRV increased significantly, while the antibody positive conversion rates were both 100%. However, after three doses of IPV, the GMTs of neutralizing antibodies against poliovirus of types Ⅰ, Ⅱ and Ⅲ increased significantly, while all the antibody positive conversion rates were 100%. No significant differences were observed in the levels of neutralizing antibodies against RV and poliovirus as well as specific Ig G against RV in various groups（P〉0. 05）. Conclusion Both IRV and IPV showed good immune effects in rats, which showed no interference when administered in combination.

关 键 词：灭活轮状病毒疫苗 灭活脊髓灰质炎疫苗 联合免疫 

分 类 号：R373.22[医药卫生—病原生物学] R392-33[医药卫生—基础医学]