脑源性神经营养因子在膀胱过度活动症无创诊断中的应用研究  

Application of brain-derived neurotrophic factor in the noninvasive diagnosis of overactive bladder

作　　者：侯瑞鹏[1] 王亚申 李健[1] 朱广博[3] 李昭夷[1] 张蓓[3] 

机构地区：[1]天津市人民医院泌尿外科,天津300121 [2]天津医科大学研究生院 [3]天津市人民医院检验科

出　　处：《临床泌尿外科杂志》2015年第4期312-315,共4页Journal of Clinical Urology

基　　金：天津市卫生局科技基金(编号2013KZ060)

摘　　要：目的：探讨脑源性神经营养因子（BDNF）在膀胱过度活动症（OAB）患者尿液中的表达及与OAB严重程度的相关性,了解BDNF在OAB无创诊断中的意义和应用价值。方法：2012年5月-2014年10月我院收治53例OAB患者（A组）,年龄18-78岁,平均（47.000±16.282）岁,其中男12例,女41例。同期选择49例无OAB相关症状且膀胱过度活动症症状评分表（OABSS）正常的健康体检者作为正常对照组（B组）。两组患者均用酶联免疫吸附法（ELISA）检测尿液中BDNF含量。A组给予口服索利那新治疗,持续用药4周后再次填写OABSS、检测尿液中BDNF含量。比较A、B两组尿液中BDNF含量、OABSS评分差异及A组用药前后尿液中BDNF含量、OABSS评分差异。每个检测样本尿液中BDNF含量值除以该样本尿肌酐值（Cr）进行标准化。结果：A组与B组BDNF/Cr分别为（1.077±0.634）和（0.454±0.231）,OABSS分别为（8.660±2.449）和（0.469±0.680）,两组比较差异有统计学意义（P=0.000）。A组治疗前后尿液中BDNF/Cr分别为（1.077±0.634）和（0.559±0.290）,OABSS评分分别为（8.660±2.449）和（3.264±1.483）,前后比较差异有统计学意义（P=0.000）。A组尿液中BDNF/Cr与OAB严重程度呈显著的相关关系（r=0.729,P=0.000）。结论：OAB患者尿液中BDNF含量明显升高,且与OAB的严重程度显著相关。经过索利那新治疗后病情缓解的OAB患者尿液中BDNF含量明显降低。BDNF可作为OAB无创诊断和疗效判定的客观指标。Objective： To explore the expression of brain-derived neurotrophic factor （BDNF） in the urine of patients with overactive bladder （OAB） and the correlations between BDNF and the severity of OAB, and to learn the significance and value of BDNF in the noninvasive diagnosis of OAB. Method.. Totally 53 cases of OAB were involved in this research from May 2012 to October 2014 in our hospital （group A）. The age was from 18 to 78 years old and the average age was （47. 000±16. 282） years old. There were 12 males and 41 females. Simultane- ously another 49 cases without OAB symptoms and with normal overactive bladder symptom score （OABSS） were selected as controls （group B）. The enzyme-linked immunosorbent assay （ELISA） was used to detect the content of BDNF in the urine. Solifenacin Was given to A group for four weeks. Then again OABSS was assessed and the BDNF in urine was tested. BDNF in urine and OABSS of A, B groups and the same parameters of A group before and after medication were compared. BDNF value in each urine sample was divided by the urine creatinine （Cr） of sample for standardization. Result： BDNF/Cr of A and B group were （1. 077±0. 634） and （0. 454±0. 231） respectively and OABSS of A and B group were （8. 660±2. 449） and （0. 469±0. 680） respectively. There were significant differences between two groups （ P = 0. 000）. BDNF/Cr in the urine of A group before and after treatment were （1. 077±0. 634） and （0. 559±0. 290） respectively and OABSS of A group before and after treatment were （ 8. 6 6 0 ± 2.4 4 9 ） and （ 3. 2 6 4 ± 1.4 8 3 ） respectively. The differences were significant （ P = 0. 0 0 0 ）. TheBI）NF/Cr in urine of A group was significantly correlated with the severity of OAB （ r = 0. 729, P = 0. 000）. Conclusion： BDNF in urine of OAB patients was significantly higher and correlated with the severity of the OAB. Moreover, BDNF in urine of OAB patients significantly reduced accompanied with the symptom remission af

关 键 词：膀胱过度活动症 脑源性神经营养因子 无创诊断 

分 类 号：R694.5[医药卫生—泌尿科学]