右美托咪定对大鼠脑缺血再灌注损伤后长期的神经保护作用  

The long-term neuroprotective effect of dexmedetomidine after focal cerebral ischemia-reperfusion injury in rats

作　　者：龚辉 张巧梅 王强[2] 

机构地区：[1]西北妇女儿童医院麻醉科,西安710003 [2]第四军医大学西京医院麻醉科,西安710032

出　　处：《神经解剖学杂志》2015年第2期138-142,共5页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(81072888)

摘　　要：目的:探讨右美托咪定对大鼠脑缺血再灌注损伤后长期的神经保护作用。方法:采用局灶性脑缺血(MCAO)模型,成年雄性SD大鼠60只,随机将其分为3组(n=20):假手术组(Sham组),缺血再灌注组(I/R组)和缺血再灌注+右美托咪定组(I/R+D组)。I/R+D组大鼠于栓塞前30min腹腔注射右美托咪定100μg/kg。检测右美托咪定对脑梗死容积、半暗带区凋亡神经元数目及神经功能的影响。结果:在大鼠缺血再灌注72 h后,TNF-α和IL-6含量明显降低(P<0.05),7 d后Tunel阳性细胞数目显著减少(P<0.01)。I/R+D组Garcia评分在3 d、7 d和14 d显著高于I/R组(P<0.05)。旷场实验发现,I/R+D组大鼠14 d中央区活动路程显著增加(P<0.01)。结论:右美托咪定可减轻大鼠脑缺血再灌注急性期的炎症反应,抑制神经元变性和凋亡,促进大鼠长期的神经功能恢复。Objective： To investigate the role of dexmedetomidine in long-term neuroprotection after cerebral ischemia reperfuision injury in rats. Methods： The animal model of local ischemia of brain was used. Sixty adult male SD rats were randomly assigned into three groups（ n = 20 each） ： sham operation group（ Sham）,ischemia-reperfusion group（ I /R） and dexmedetomidine ＋ ischemia-reperfusion group（ I / R ＋ D）,rats in I / R ＋ D group were intraperitoneally injected with 100 μg / kg dexmedetomidine before ischemia. The infarction volume,brain water content,neuronal apoptosis,Garcia score and open-field test were observed for assessing neurologic injury and function recovery. Results： seventy two hour after ischemia-reperfasion,the expression of TNF-α and IL-6 were significantly decreased（ P 〈 0. 05）. Ater 7 d of ischemia-reperfusion the number of Tunel-positive cells was reduced in I / R ＋ D group. Moreover,Garcia score was significantly increased at the 3 d,7 d and 14 d day（ P 〈 0. 05） in I / R ＋ D group after reperfusion. In Open-field test,the distance in the central area was significantly increased（ P 〈 0. 01） compared with that in I / R group at the 14 th day after reperfusion. Conclusion： Dexmedetomidine decreased inflammatory response,inhibited neuronal degeneration and apoptosis in acute phase after ischemia injury,improved long-term neurologic function recovery after cerebral ischemia-reperfusion injury.

关 键 词：脑缺血再灌注损伤 炎症反应 神经元变性 凋亡 神经功能 大鼠 

分 类 号：R614[医药卫生—麻醉学]