磁共振T1ρ成像检测兔膝关节软骨早期退变  

T1ρ magnetic resonance imaging for detection of early articular cartilage degeneration in knees of rabbit models

作　　者：王昊[1] 沈思[2] 张景[2] 史长征[2] 刘斯润[2] 

机构地区：[1]暨南大学附属第一医院疼痛门诊,广东广州510630 [2]暨南大学附属第一医院影像中心,广东广州510630

出　　处：《中国医学影像技术》2015年第4期488-492,共5页Chinese Journal of Medical Imaging Technology

基　　金：暨南大学科研培育与创新基金青年基金(21612304)

摘　　要：目的探讨磁共振T1ρ成像检测兔膝关节软骨早期退变的可行性。方法将32只实验兔随机分组;其中24只(实验组)随机分为A^D组,每组6只,均予左侧膝关节腔内注射木瓜蛋白酶,建立关节软骨早期退变的模型;余8只作为空白对照组(E组),随机分为EA、EB、EC、ED亚组,每组2只,分别做为A、B、C、D组的空白对照。于末次注药后1、2、3、4周分别对A^D组(对EA、EB、EC、ED亚组进行同期对照成像)行双膝常规T2W、3D-FS-SPGR和T1ρ扫描后处死并取双膝关节股骨髁软骨做组织学检查。测量并分析兔股骨髁软骨T1ρ值组内及组间的差异,并与组织学结果对照。结果实验组处理侧(左侧)股骨髁软骨T1ρ值均明显高于对照侧(P<0.05);实验组处理侧股骨髁软骨T1ρ值随时间推移逐渐升高(P<0.05);T1ρ伪彩图显示实验组处理侧软骨呈浅绿色向深绿色的渐进性改变;组织学检查显示实验组处理侧股骨髁软骨发生早期退变,软骨内PG含量明显下降。结论 T1ρ值能够敏感地量化反映关节软骨退变早期、形态学改变之前的蛋白多糖变化。T1ρ成像可能为临床检测关节软骨早期退变提供影像学依据。Objective To explore the feasibility of T1ρmagnetic resonance imaging for detection of early articular cartilage degeneration of rabbit models.Methods A total of 24 rabbits were randomly divided into four experimental groups（group A,B,C,D,each n=6）to establish articular cartilage models at different stage of early degeneration by injecting papain into the left knee joint cavity,and 8rabbits were considered as blank control（group E）,and then randomized into four subgroups（EA,EB,EC,ED,each n=2）.The T1ρ,3D-FS-SPGR and T2 Wimages of the bilateral knee joints were obtained for 32 rabbits using a 3.0T MRI.Group A,B,C,D were scanned in sequence after one to four week from the last injection,and EA,EB,EC,EDunderwent the same sequence scanning during the period.A total of 32 rabbits were executed after scanning and the femoral condyle cartilage were histologically examined.T1ρvalues of the femoral condyle cartilage were measured and statistically analyzed,and compared with the histologic results.Results T1ρvalues of the experimental side（left side）were significantly higher than that of the control side（right side,P〈0.05）.T1ρvalues of the experimental side increased gradually with the passage of time（P〈0.05）.T1ρpseudo color map of the experimental side showed a tale of gradual change from light green to dark green,histological examination demonstrated the proteoglycan content of the experimental side decreased,and indicated the occurrence of early degeneration.Conclusion T1ρvalues can sensitively and quantitatively reflect the changes of proteoglycans within articular cartilage before the morphologic alterations,therefore T1ρcould potentially act as a clinical tool to assess the prophase cartilage degeneration.

关 键 词：磁共振成像 T1ρ 关节软骨 早期退变 蛋白多糖 

分 类 号：R445.2[医药卫生—影像医学与核医学] R-332[医药卫生—诊断学]