早期运动训练对脊髓损伤大鼠后肢运动功能影响及相关机制研究  

Effects of early exercise training on hind limbs motor function in rats after spinal cord injury and the related mechanism

作　　者：李萌[1] 陈银海[1] 张慧[1] 黄玉琴[1] 

机构地区：[1]南方医科大学珠江医院门诊部,广州510282

出　　处：《中国康复医学杂志》2015年第4期318-323,共6页Chinese Journal of Rehabilitation Medicine

基　　金：广东省科技计划项目(2010B080701020)

摘　　要：目的:探讨早期运动训练对脊髓损伤大鼠后肢运动功能的影响及其相关机制。方法:成年SD雄性大鼠75只,采用改良钳夹法建立大鼠T10损伤模型。术后随机分为假手术组(A组)、脊髓损伤组(B组)、脊髓损伤+跑台训练组(C组),术前3天及术后第2、3、7、14天采用BBB行为功能评定量表对大鼠后肢运动功能进行评分。术后第2、3、5、7天取损伤中心头尾端约1cm的脊髓组织进行脊髓含水量的测定,术后第3天行免疫组化检测脊髓组织损伤段半胱氨酸蛋白酶3(caspase-3)和髓过氧化物酶(MPO)的表达情况,行HE染色观察脊髓组织病理学改变。结果:1术前各组大鼠后肢运动功能评分均为21分,术后B组和C组大鼠评分随着时间的延长而逐渐升高,第7天时3组之间差异有显著性意义(P<0.05)。2A组大鼠脊髓含水量基本不变,脊髓损伤后第3天,水肿达到高峰,随后逐渐下降,到术后第7天脊髓含水量仍高于A组,且各个时间点差异均有显著性意义(P<0.05)。3术后第3天A组大鼠caspase-3少量表达,MPO基本不表达,B组大鼠caspase-3和MPO的表达均高于C组,3组相比差异均有显著性意义(P<0.05)。4B组脊髓组织病理学改变较C组重。结论:早期运动训练可以促进脊髓损伤大鼠后肢运动功能的恢复,其机制可能与减轻脊髓损伤后水肿,减少细胞凋亡和炎症反应有关。Objective： To explore the influence of early exercise training on motor function of hind limbs in rats after spi- nal cord injury（SCI） and the related mechanism. Method： Seventy-five adult male SD rats were used to establish the model of T10 injury in rats using the modified clamping method. They were randomly divided into sham operation group （group A）, SCI group （group B） and SC1 with treadmill training group （group C）. On 3 days before operation and 2, 3, 7, 14 days after SCI, BBB scale was used to evaluate hind limbs function of rats. The spinal cord tissue at injury centre about l cm length was taken off to determine the water content of spinal cord on 2, 3, 5 and 7 days after inju- ry. The expressions of cysteine proteinase 3 （caspase-3） and myeloperoxidase （MPO） were detected by immuno- histochemical technique and HE staining was used to observe pathological change of spinal cord tissue on the 3rd postoperative day. Result： ①Before operation BBB scores of rats in 3 groups all were 21, after operation hind limbs motor func-tion BBB scores in group B and group C increased gradually with the extension of time. There were signifi- cant differences among three groups on the 7th postoperative day （P〈 0.05）. ② The water content of spinal cord in group A basically remained unchanging, on the 3rd postoperative day, edema reached the peak and then gradually decreased, water content on the 7th postoperative day was higher than that in group A. At each time point the differences were statistically significant （P 〈 0.05）. ③ At the 3rd post operative day in group A caspase-3 had little expression and MPO had almost no expression. In group B caspase-3 and MPO expres- sions were higher than that of group C. In three groups the differences had statistical significance （P 〈 0.05）. ④ In group B pathological change of spinal cord tissue was severer than that in group C. Conclusion： Early exercise training can enhance the recovery of hind limbs motor function in rats af

关 键 词：脊髓损伤 跑台训练 炎症 水肿 凋亡 

分 类 号：R87[医药卫生—运动医学]