针刀用于腰椎间盘突出症根性神经痛大鼠中枢镇痛的机制研究  

Effects of acupotomy on central analgesic mechanism of radicular pain of lumbar disc herniation in rats

作　　者：崔成埈 郭长青[1] 付伟涛[1] 梁楚西 黄怡然[1] 孙红梅[2] 许红[2] 郭健[2] 赵淑英[2] 张丽萍[1] 朴起范 

机构地区：[1]北京中医药大学针灸推拿学院,北京100029 [2]北京中医药大学基础医学院

出　　处：《中国康复医学杂志》2015年第4期324-328,共5页Chinese Journal of Rehabilitation Medicine

基　　金：北京中医药大学自主课题项目(53210100604209)

摘　　要：目的:观察针刀疗法对腰椎间盘突出症根性神经痛大鼠下丘脑和脊髓中5-羟色胺、β-内啡肽含量的影响,探讨针刀疗法对腰椎间盘突出症根性神经痛干预作用的可能机制。方法:将40只雄性SD大鼠随机分为正常组、假模型组、模型组、针刀组,共4组,每组10只。用热痛测试仪检测各组大鼠造模前后的缩爪潜伏期;HE染色观察L5神经根的病理学变化;造模后第8天分别取各组大鼠下丘脑和脊髓,采用双抗夹心ELISA方法检测下丘脑和脊髓中5-羟色胺、β-内啡肽的含量。结果:模型组下丘脑和脊髓中5-羟色胺含量较正常组显著降低,差异有显著性意义(P<0.05),针刀组较模型组显著升高,差异有显著性意义(P<0.05);模型组β-内啡肽含量较正常组显著升高,差异有显著性意义(P<0.01),针刀组较模型组显著降低,差异有显著性意义(P<0.05)。结论:针刀治疗可调节中枢疼痛相关递质5-羟色胺和β-内啡肽的合成和分泌,降低中枢痛觉传递和痛觉过敏,从而有效地发挥了镇痛作用。Objective： To observe effects of acupotomy on 5-hydroxytryptamine（5-HT） and β-endorphin（β-EP） in hypothalamus and spinal cord of rats with discogenic radiculalgia, so as to explore possible mechanism of acupotomy.Method： Forty male SD rats were randomly divided into four groups： normal group, sham model group, model group and acupotomy group, 10 rats in each group. Samples were taken on the 8th d after modeling. The hypothalamus and spinal cord were taken to observe the changes of 5-HT and β-EP by ELISA.Result： In model group, 5- HT in hypothalamus and spinal cord decreased significantly（P〈0.05） compared with normal group, in acupotomy group those increased compared with model group（P〈0.05）; In model group, β- EP in hypothalamus and spinal cord increased significantly（P〈0.05） compared with normal group,in acupotomy group those decreased compared with model group（P〈0.05）.Conclusion： Acupotomy had benign effects on regulating 5-HT and β-EP in hypothalamus and spinal cord of rats with lumbar disc herniation and reducing central algotransmission and hyperalgia, so as to provide analgesic effect.

关 键 词：针刀疗法 腰椎间盘突出症 根性神经痛 5-羟色胺 Β-内啡肽 

分 类 号：R245[医药卫生—针灸推拿学]