山东省部分地区仔猪病毒性腹泻流行病学调查  

Epidemiological investigation of piglet diarrhea virus disease in Shandong province

作　　者：薛瑞雪[1] 田野[1] 田夫林[2] 张月[1] 孙振[1] 陈超[1] 姜世金[1] 陈书民[2] 

机构地区：[1]山东农业大学动物医学学院,山东泰安271018 [2]山东省动物疫病预防与控制中心,山东济南250022

出　　处：《中国预防兽医学报》2015年第4期254-257,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：山东省仔猪腹泻流行病学调查和综合防控技术研究(2013GGC15003)

摘　　要：为了解山东省仔猪病毒性腹泻的流行状况,本研究根据Gen Bank中登录的猪流行性腹泻病毒（PEDV）、猪传染性胃肠炎病毒（TGEV）及猪轮状病毒（PRo V）序列,分别设计扩增引物,采用RT-PCR方法对2013年10月～2014年10月采集的226份腹泻样品进行检测。结果显示226份病料样品中,PEDV、TGEV及PRo V的阳性率分别为34.96%、2.21%及28.76%,并且不同季节PEDV和PRo V均有感染,表明PEDV和PRo V是引起山东省仔猪腹泻的主要病原,并且山东省PRo V的感染率明显高于全国。规模化猪场与散养户相比,PEDV、TGEV及PRo V 3种病原的检出率明显降低,而且相对于未免疫腹泻二联苗的母猪,免疫母猪生产的仔猪发生腹泻后,该病原的检出率有所降低,表明合理的饲养环境和免疫对该病具有有效的防控作用。To study the epidemiology of piglets diarrhea virus infection, a total of 226 clinical samples were collected and subjected to detection of porcine epidemic diarrhea virus （PEDV）, transmissible gastroenteritis virus （TGEV） and porcine rotavirus （PRoV） by RT-PCR with the primers designed according to the sequences of these viruses reference strains in GenBank. The results showed that positive rates of PEDV, TGEV and PRoV were 34.96%, 2.21% and 28.76%, respectively, and the infection rate of PRoV was significantly higher than that of average rate in other provinces. The prevalence of PEDV and PRoV were different with seasons, which indicated that PEDV and PRoV were the major pathogens for the piglet diarrhea. Compared with free-range reared pigs, the large-scale pig farms had low positive rates of the three viruses. Meanwhile, after with the vaccination practices, the piglet infected rates of the three viruses were lower than that in non-immunized piglets, which indicated that reasonable feeding management and vaccination practices have added benefit of the prevention of the piglet diarrhea.

关 键 词：猪流行性腹泻病毒 猪传染性胃肠炎病毒 猪轮状病毒 流行病学调查 

分 类 号：S852.65[农业科学—基础兽医学]