D型产气荚膜梭菌类毒素疫苗的研制及保护效果的评价  

Development and evaluation of Clostridium perfringens type D toxoid vaccine

作　　者：刘雪慧[1] 王方山 樊金[1] 郭梦娇[1] 艾文豪[1] 秦贵平[1] 吴洁[1] 刘萌萌[1] 王海荣[1] 柴同杰[1] 

机构地区：[1]山东农业大学动物科技学院,山东泰安271018

出　　处：《中国预防兽医学报》2015年第4期310-314,共5页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家自然科学基金(31270172);国家科技支撑项目课题(2012BAD39B02)

摘　　要：为研制更有效的预防家畜D型产气荚膜梭菌肠毒血症的疫苗,本研究采用D型产气荚膜梭菌标准菌株增菌培养,经自制产毒培养基高效产毒,制备成D型产气荚膜梭菌氢氧化铝类毒素疫苗和白油类毒素疫苗。通过不同剂量疫苗免疫绵羊的攻毒保护试验和免疫绵羊的抗体水平监测,评估疫苗的免疫保护效果。结果表明:D型产气荚膜梭菌类毒素疫苗安全性良好,氢氧化铝苗和白油苗对绵羊有效免疫剂量均为4 m L(外毒素对小鼠半数致死量为2-6.4/m L),绵羊接种一个有效免疫剂量后,氢氧化铝疫苗免疫保护周期为6周以上;白油疫苗免疫保护周期为21周以上。本研究结果表明两种疫苗均具有良好的应用前景。To develop effective vaccine against enterotoxemia caused by Clostridium perfringens type D, toxins were efficiently produced from the standard strain in the modified toxin-producing medium, and two kinds of toxoid vaccines were prepared with the aluminum hydroxide and white oil emulsification, respectively. Sheep were immunized with different dose of aluminum hydroxide toxoid vaccine and white oil toxoid vaccine respectively, and the effective immune dose was determined by challenge test. Immune protective efficacy was evaluated by detecting the antibody level. The results showed that the toxiod vaccines was safety, and the effective immune dose of the two vaccines was 4 mL （LD_50 of the toxins was 2^-6.4/mL in mice）. The aluminum hydroxide toxoid vaccine protect the sheep from enterotoxemia for more than 6 weeks, while the toxoid oil emulsion vaccine protect for more than 21 weeks. Both of the vaccines had potential application prospects.

关 键 词：产气荚膜梭菌 类毒素疫苗 氢氧化铝佐剂 白油佐剂 抗体消长规律 

分 类 号：S852.61[农业科学—基础兽医学]