用DNAStar软件预测Rv1410c结核分枝杆菌蛋白抗原表位  

Prediction of epitopes of Rv1410c Mycobacterium tuberculosis protein using DNAStar software

作　　者：李江英[1,2] 白雪娟[2] 梁艳[2] 张俊仙[2] 阳幼荣[2] 赵卫国[1,2] 吴雪琼[2] 

机构地区：[1]河北北方学院,河北张家口075000 [2]解放军第309医院全军结核病研究所全军结核病防治重点实验室,北京100000

出　　处：《细胞与分子免疫学杂志》2015年第4期474-477,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家重大传染病防治科技重大专项基金(2012ZX10003008002);军队医学科技"十二五"重点项目(BWS11J050);北京市科技创新基地培育与发展工程专项(Z141107004414021)

摘　　要：目的预测Rv1410c结核分枝杆菌（MTB）的抗原表位。方法利用DNAStar软件包中Editseq软件将Rv1410c MTB氨基酸序列进行编辑保存,然后利用Protean软件进行氨基酸序列分析,预测Rv1410c MTB的二级结构、B细胞抗原表位及T细胞抗原表位,然后再用BLAST分析其与人类抗原表位的同源性。结果 Rv1410c具有丰富的二级结构,含有较多潜在的B细胞抗原肽表位,主要位于1~10、67~77、129~137、189~205、222~235、253~267、296~306、330~338、359~367、462~467、494~517氨基酸残基或其附近,这些区域基本上含有β转角结构,亲水性、表面可能性和柔韧性指数都较高;也含有较多潜在的T细胞表位,主要位于16~37、84~102、108~115、137~160、202~207、219~222、245~263、321~325、355~362、387~391、417~445、486~494氨基酸残基或其附近。结论 Rv1410c MTB既含有较多潜在的B细胞抗原表位,也含有较多潜在的T细胞抗原表位。Objective To predict the epitopes of Rv1410c Mycobacterium tuberculosis（MTB） protein.Methods Rv1410c MTB amino acid sequence was saved and edited using Editseq software of DNAStar package,and then Protean software was used to analyze the amino acid sequence and predict the secondary structures,B cell epitopes and T cell epitopes of Rv1410 c MTB.Finally,BLAST was utilized to assess its homology with human epitopes.Results The Rv1410c MTB protein had abundant secondary structures.It contained a higher proportion of potential B cell epitopes located at 1-10,67-77,129-137,189-205,222-235,253-267,296-306,330-338,359-367,462-467,494-517 amino acid residues or nearby.Theses epitopes contained beta angle structure mostly,presenting with better antigenicity,higher surface probability and flexibility.There were also several potential T cell epitopes located at 16-37,84-102,108-115,137-160,202-207,219-222,245-263,321-325,355-362,387-391,417-445,486-494 amino acid residues or nearby.Conclusion Rv1410c MTB protein contains both potential B cell antigen epitopes and potential T cell antigen epitopes.

关 键 词：抗原表位 DNAStar软件 结核分枝杆菌 Rv1410c 二级结构 

分 类 号：R857.3[医药卫生—航空、航天与航海医学] Q518.1[医药卫生—临床医学]