丁酸钠下调CNE2鼻咽癌细胞IFN-γ诱导的吲哚胺2,3双加氧酶表达并促进其乙酰化和泛素化  

Sodium butyrate down-regulates IFN-γ-induced indoleamine 2,3 dioxygenase and promotes its acetylation and ubiquitination in CNE2 nasopharyngeal carcinoma cells

作　　者：王霞[1] 张秋桂[2] 邱瑜[3] 谢婉莹[2] 张凯华[1] 江冠民[4] 

机构地区：[1]南华大学附属第二医院输血科,湖南衡阳421001 [2]南华大学附属第一医院检验科,湖南衡阳421001 [3]湖南省儿童医院ICU科,湖南长沙410007 [4]中南大学湘雅医学院附属肿瘤医院检验科,湖南长沙410013

出　　处：《细胞与分子免疫学杂志》2015年第4期487-490,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：湖南省自然科学基金(13JJ4078)

摘　　要：目的探讨丁酸钠(NaB)对CNE2鼻咽癌细胞γ干扰素(IFN-γ)诱导的吲哚胺2,3双加氧酶(IDO)表达调控及分子机制。方法采用Western blot法、免疫共沉淀等方法检测鼻咽癌细胞内IDO的表达及乙酰化、泛素化修饰情况。结果与单独加IFN-γ对照组相比,Western blot结果显示,IFN-γ和Na B联合处理的CNE2细胞IDO蛋白表达增加;免疫共沉淀实验结果表明,联合处理CNE2细胞乙酰化IDO水平和泛素化IDO水平均明显增高;与NaB和IFN-γ联合处理的CNE2细胞相比,NaB、IFN-γ和硼替佐米(bortezomib)联合处理的CNE2细胞的IDO表达量增加。结论 NaB能以剂量依赖性的方式下调鼻咽癌细胞内IFN-γ诱导的IDO表达,IFN-γ和Na B联合处理促进IDO的乙酰化和泛素化。Objective To investigate molecular mechanisms of posttranslational regulation of sodium butyrate（NaB） on indoleamine 2,3-dioxygenase（IDO） expression induced by interferon γ（IFN-γ） in CNE2 nasopharyngeal carcinoma cells.Methods Western blotting and co-immunoprecipitation assay were used to detect the expression,acetylation and ubiquitination of IDO in CNE2 nasopharyngeal carcinoma cells.Results Western blotting demonstrated that while the cells were treated with IFN-γ and NaB compared with IFN-γ treatment only,the expression of IDO protein increased significantly; furthermore,in the co-immunoprecipitation assay,while the cells were treated with IFN-γ and NaB compared with the ones treated with IFN-γ only,acetylation and ubiquitination of IDO increased significantly.Western blotting showed that the cells treated with NaB and IFN-γ presented with lower IDO protein expression than the ones treated with NaB,IFN-γ and bortezomib simultaneously.Conclusion NaB could down-regulate the expression of IDO induced by IFN-γ in a dose-dependent manner,and the combined treatment of IFN-γ and NaB could promote the acetylation and ubiquitination of IDO in CNE2 nasopharyngeal carcinoma cells.

关 键 词：丁酸钠 吲哚胺2 3双加氧酶 肿瘤免疫耐受 乙酰化 泛素化 

分 类 号：R392.11[医药卫生—免疫学] R965[医药卫生—基础医学]