HLA-E基因多态性及血浆可溶性HLA-E水平与乳腺癌遗传易患性的关系  

HLA-E gene polymorphisms and plasma soluble HLA-E levels and their relationship with genetic susceptibility to breast cancer

作　　者：周艳[1] 吴振村 唐玉红[1] 贾天军[1] 

机构地区：[1]河北北方学院免疫教研室,河北张家口075000 [2]张家口市妇幼保健院检验科,河北张家口075000

出　　处：《细胞与分子免疫学杂志》2015年第4期524-527,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：河北省教育厅重点科研项目(Z2011208);河北北方学院青年基金(2013年);张家口市科技项目(1321062D)

摘　　要：目的探讨人类白细胞抗原E(HLA-E)基因多态性及血浆可溶性HLA-E(sHLA-E)水平与中国张家口地区汉族女性乳腺癌遗传易患性的关系。方法采用聚合酶链反应-序列特异性引物(PCR-SSP)法对中国张家口地区200例乳腺癌患者和228例健康对照人群HLA-E等位基因进行检测;同时用ELISA检测乳腺癌患者和健康对照人群sHLA-E水平。结果乳腺癌患者和健康对照组均检出2种等位基因:HLA-E*0101和HLA-E*0103;3种基因型:HLA-E*0101/HLA-E*0101、HLA-E*0101/HLA-E*0103和HLA-E*0103/HLA-E*0103。其中HLA-E*0103等位基因和HLA-E*0103/HLA-E*0103基因型在乳腺癌患者组的分布频率明显高于健康对照组,且携带HLA-E*0103/HLA-E*0103基因型的个体乳腺癌发病风险明显增加;血浆sHLA-E水平在乳腺癌患者组和健康对照组分布无明显差异,进一步分析发现携带HLA-E*0103/HLA-E*0103基因型患者血浆sHLA-E水平明显高于健康对照组;且HLA-E基因多态性及血浆sHLA-E水平与乳腺癌临床分期无明显相关性。结论HLA-E基因多态性与中国张家口地区汉族女性乳腺癌遗传易患性相关;HLA-E*0103/HLA-E*0103基因型可能是中国张家口地区汉族女性乳腺癌的易感基因;而血浆sHLA-E水平可能与乳腺癌的遗传易患性无明显相关性。Objective To investigate the association of human leukocyte antigen-E（HLA-E） gene polymorphisms and plasma soluble HLA-E（s HLA-E） levels with genetic susceptibility to breast cancer in Zhangjiakou women of Han nationality.Methods HLA-E typing was performed in 200 patients with breast cancer and 228 healthy controls from Zhangjiakou area by means of polymerase chain reaction-sequence-specific priming（PCR-SSP）.Simultaneously,s HLA-E was detected by ELISA.Results We found two alleles of HLA-E： HLA-E＊ 0101 and HLA-E＊ 0103,and three genotypes,namely HLA-E＊0101 / HLA-E＊ 0101,HLA-E＊ 0101 / HLA-E ＊ 0103 and HLA-E ＊ 0103 / HLA-E ＊ 0103.The frequencies of HLA-E ＊ 0103 allele and HLA-E＊ 0103 / HLA-E＊ 0103 genotype showed a significant difference between healthy controls and patients with breast cancer.The HLA-E＊ 0103 / HLA-E＊ 0103 genotype significantly increased the risk for breast cancer.Plasma levels of s HLA-E showed no significant difference in breast cancer patients and controls.While in breast cancer patients with HLA-E ＊ 0103 /HLA-E＊ 0103 genotype,plasma levels of s HLA-E were significantly elevated compared with those in healthy controls.HLA-E genotypes and s HLA-E levels had no obvious relationship with tumor grading.Conclusion HLA-E gene polymorphisms are associated with the development of breast cancer in Zhangjiakou women of Han nationality.HLA-E ＊ 0103 / HLA-E ＊ 0103 genotype is probably susceptible genotype for breast cancer,while plasma s HLA-E level probably has no association with breast cancer susceptibility.

关 键 词：HLA-E 聚合酶链反应-序列特异性引物 乳腺癌 多态性 

分 类 号：R392.13[医药卫生—免疫学] R737.9[医药卫生—基础医学]