重组鸡痘病毒rFPV_(Hg-Hp)理化特性检测  

Physical and Chemical Factors Detection of the Recombinant Fowlpox Virus rFPV_(Hg-Hp)

作　　者：刘继[1] 李昌[2] 朱羿龙[2] 朱光泽[1] 张艳芳[1] 杜寿文[2] 谭鹏[2] 李一权 郭焱[2] 李金泽[1] 邢彬[2] 金宁一[2] 

机构地区：[1]长春中医药大学,吉林长春130117 [2]军事医学科学院军事兽医研究所,吉林长春130122

出　　处：《生物技术通讯》2015年第2期223-226,共4页Letters in Biotechnology

基　　金：国家科技重大专项(2012ZX10001005-006);国家自然科学基金(31472197);中国博士后科学基金(20110491803;2012T50859)

摘　　要：目的：检测各理化因素对重组鸡痘病毒rFPVHg-Hp的影响，为临床前研究奠定基础。方法：将重组鸡痘病毒rFPVHg-Hp经热、酸、碱、乙醚、氯仿、胰蛋白酶、超声破碎、紫外线照射处理后，接种于CEF细胞，通过测定TCID50观察重组鸡痘病毒rFPVHg-Hp毒力活性的变化，由此分析这些理化因素对重组鸡痘病毒rFPVHg-Hp的影响。结果与结论：重组鸡痘病毒rFPVHg-Hp不耐高温，在55℃作用45 min或60℃作用15 min即可完全灭活；但是其对酸和乙醚具有一定的耐受性，在pH值为3-9的范围内，pH值的变化对病毒滴度无明显影响；同时紫外线照射无法使得培养基中的病毒粒子完全灭活；该病毒对氯仿、胰蛋白酶较为敏感，二者均可使病毒滴度下降；在适应的条件下，重组鸡痘病毒rFPVHg-Hp对超声波具有一定的耐受性。Objective： To detect the influence of rFPVHg-Hp which was disposed by physical and chemical factors and provide a foundation for the following clinical study. Methods： The CEF cells were infected by the rFPVHg-Hp which was disposed by heat, acid-base, ether, chloroform, trypsin, ultrasonic waves and ultraviolet rays, and then inspected the changes in virus titer to analyze the influence of rFPVHg-Hp which was disposed by these physical and chemical factors. Results Conclusion： The results showed that rFPVHg-Hp was sensitive to heat. It was completely inactivated by 55℃, 45 min or 60℃, 15 min. rFPVHg-Hp was tolerant of acid-base and ether, and the titer of rFPVHg-Hp was not significantly affected when the pH was changed in a range of 3-9. The virion in the medium was not completely inactivated by ultraviolet rays, and the titer of rFPVHg-Hp was reduced by chloroform and trypsin. rFPVHg-Hp was tolerant of ultrasonic waves in the right conditions.

关 键 词：重组鸡痘病毒 理化因子 病毒滴度 

分 类 号：Q503[生物学—生物化学]