肺炎支原体流行株基因分型研究  

Genotyping analysis of epidemic strains of Mycoplasma pneumoniae in Suzhou area

作　　者：李东[1,2] 范丽萍[1] 孙惠泉[1] 郝创利[1] 邵雪军[3] 徐俊[3] 

机构地区：[1]苏州大学附属儿童医院呼吸科,江苏苏州215003 [2]滨州医学院附属医院儿科,山东滨州256603 [3]苏州大学附属儿童医院检验科,江苏苏州215003

出　　处：《临床儿科杂志》2015年第4期338-341,共4页Journal of Clinical Pediatrics

基　　金：苏州市社会科技发展项目(No.sys201214)

摘　　要：目的了解肺炎支原体(MP)感染患儿MP流行株基因分型及其流行趋势变化。方法采集2012年2月至2013年12月住院MP感染患儿的鼻咽分泌物,采用基于MP P1基因的巢式PCR及多重PCR方法进行MP基因亚型的监测,并分析其流行趋势。结果在313例标本中检出P1-I型304例(97.12%),P1-II型8例(2.56%),V2型变异株1例(0.32%),基因测序结果与巢式多重PCR结果相符合。结论巢式多重PCR方法能够直接有效地对MP进行P1基因分型,研究期间MP感染株以P1-I型为主,发现1例V2变异株。ObjectiveTo analyze the genotype and variation ofMycoplasma pneumonia （MP） strains isolated from chil-dren with MP infection in Soochow area.MethodsThe nasopharyngeal secretions from hospitalized children with MP infection were collected during January 2012 and December 2013. The nested-multiplex PCR based on MPP1 gene was performed to detect the subtype ofMP gene.ResultsIn 313 samples, 304 （97.12 %） samples were classiifed as P1-I type and 8 （2.56%） sam-ples were classiifed as P1-II type and one （0.32%） was V2 variant. Gene sequencing results were consistent with nested-multiple PCR results.ConclusionsNested-multiplex PCR is a reliable method for genotyping of MPP1 gene. During the study period, P1-I type was the common genotype and only one case of V2 variant was found.

关 键 词：肺炎支原体 P1基因 基因分型 

分 类 号：R725.6[医药卫生—儿科]