儿童产超广谱β-内酰胺酶大肠埃希菌Ⅰ类整合子及其与ESBLs基因关系的研究  

Class Ⅰ integron and its correlation with genes coding for ESBLs in ESBLs-producing Escherichia coli from children

作　　者：蒋鸿超[1] 奎莉越[1] 黄海林[1] 苏敏[1] 樊茂[1] 

机构地区：[1]昆明医科大学附属儿童医院检验中心,江南昆明650000

出　　处：《临床儿科杂志》2015年第4期345-347,共3页Journal of Clinical Pediatrics

摘　　要：目的研究儿童产超广谱β-内酰胺酶(ESBLs)大肠埃希菌Ⅰ类整合子分布及其与ESBLs基因的相关性。方法采用PCR方法检测100株产ESBLs大肠埃希菌的ESBLs基因,以及100株产ESBLs与100株非产ESBLs大肠埃希菌的Ⅰ类整合子基因,分析Ⅰ类整合子与细菌耐药性的关系以及与ESBLs基因的关系。结果儿科临床产ESBLs大肠埃希菌基因分型以CTX-M型最多见(84.0%),其次为TEM-1型(63.0%);产ESBLs菌株基因分型分布以TEM-1合并CTX-M型最多见(45.0%),其次为CTX-M型(34.0%);产ESBLs和非产ESBLs菌株中Ⅰ类整合子扩增阳性例数分别是100例(100%)和25例(25%),差异有统计学意义(P<0.05);Ⅰ类整合子阳性的菌株耐药性明显高于Ⅰ类整合子阴性的菌株,阳性株对环丙沙星、左氧氟沙星以及氨基糖苷类耐药比率较高,依次为86.4%、88.0%和80.0%。结论Ⅰ类整合子在儿科产ESBLs大肠埃希菌中的分布明显高于非产ESBLs菌株,并与产ESBLs菌株耐药性形成高度相关。ObjectiveTo study the correlation with Genes Coding forESBLs and ClassⅠIntegron in ESBLs-producing Escherichia coli from children.MethodsPCR was used for gene typing detection of 100 strains of ESBLs-producingEsche-richia colistrains. While detection of class I integrase gene in the 100 strains ESBLs-producingEscherichia coli and 100 strains of non-ESBLs producingEscherichia coli were separately performed by PCR. It provides the solid base not only to reveal the relationship between class I integron and drug resistance, but also the relationship between class I integron and ESBLs-producing. ResultsThe most frequently genotyping from ESBLs-producingEscherichia coli in children isCTX-M （84%）, followed by TEM-1（63%）. The predominant distribution of genotype in ESBL- producing strains isTEM-1 ＋CTX-M （45%）, followed by CTX-M （34%）. Class I integrase gene detected in ESBLs- producing and non- ESBLs producing strain were 100 cases （100%） and 25 cases （25%） separately, the difference was statistically signiifcant （P〈0.05）; drug resistance in class I integron positive strains were signiifcantly higher than in class I integron negative strains, especially in Ciprolfoxacin, Levolfoxacin, and Amino-glycoside （86.4%, 88%, and 80%）.ConclusionsThe distribution of Class I integron in ESBLs-producingEscherichia coli is signiifcantly higher than that in non-ESBLs-producing strains, It is rational that Class I integron highly correlate with strong drug resistance in ESBLs-producing strains.

关 键 词：整合子 耐药基因 超广谱Β-内酰胺酶 儿童 

分 类 号：R446.5[医药卫生—诊断学]