冬凌草甲素对棕榈酸诱导的内皮细胞损伤的保护作用  

Protection of Oridonin on Endothelial Cells from Injury Induced by Palmitic Acid

作　　者：胡雪梅[1] 刘洁[1] 孙力[1] 石小丽[1] 陈静[1] 黄琦[1] 徐焱成[1] 

机构地区：[1]武汉大学中南医院内分泌科,湖北武汉430071

出　　处：《武汉大学学报（医学版）》2015年第3期329-331,348,共4页Medical Journal of Wuhan University

基　　金：国家自然科学基金面上项目(编号:81170769;81370872);湖北省自然科学基金面上项目(编号:2013CF8243)

摘　　要：目的:探讨冬凌草甲素(oridonin)对棕榈酸诱导的人脐静脉内皮细胞(HUVECs)损伤的保护作用。方法:体外培养HUVECs,噻唑蓝法(MTT)确定冬凌草甲素作用的最佳浓度和时间;细胞分为空白对照组、棕榈酸组、棕榈酸+oridonin预处理组(以下简称"oridonin预处理组",棕榈酸浓度为500μmol/L),oridonin预处理24h,棕榈酸作用48h。流式细胞仪检测细胞凋亡率,硝酸还原酶法检测各组细胞上清液中一氧化氮(NO)的浓度。结果:冬凌草甲素作用于HUVECs细胞的最佳时间是24h,最佳浓度为5μmol/L。与空白对照组比较,棕榈酸组HUVECs细胞凋亡率[(21.23±4.11)%vs(1.63±1.23)%,t=7.90,P<0.01]明显增加,上清液中NO的浓度[(114.67±17.01)μmol/Lvs(242.33±20.11)μmol/L,t=-8.396,P<0.01]明显降低。相对于棕榈酸组,oridonin预处理组HUVECs凋亡率明显降低[(7.77±2.57)%vs(21.23±4.11)%,t=-4.81,P<0.01],上清液中NO的浓度[(191.33±13.01)μmol/L vs(114.67±17.01)μmol/L,t=6.20,P<0.01]明显升高。结论:冬凌草甲素能抑制棕榈酸诱导的HUVECs细胞凋亡,并增加NO的浓度。Objective：To study the protective effect of oridonin on vascular endothelial cells from injury induced by palmitic acid.Methods：The optimum time and concentration of oridonin on Human umbilical vein endothelial cells（HUVECs）were determined by MTT assay.The model of HUVECs was established and divided into three groups：control group,palmitic acid group,and oridonin group.In the oridonin group,HUVECs were pretreated with 5μmol/L oridonin under the condition of 500μmol/L palmitic acid,while other groups received 500μmol/L palmitic acid or dimethyl sulfoxide（DMSO）.After 48 htreatment,the cell apoptosis was detected by flow cytometry while the concentration of NO in the supernatant was measured by using nitrate reductase method.Results：The optimum time of oridonin treating on HUVECs was 24 h,and the optimum concentration was 5 μmol/L.Compared with the control group,the apoptosis rate（21.23%±4.11% vs 1.63% ±1.23%,t=7.90,P〈0.01）was significantly higher while the concentration of NO in the supernatant was significantly lower in the HUVECs in the palmiticacid group as（114.67±17.01）μmol/L vs（242.33±20.11）μmol/L（t=-8.396,P〈0.01）.But the apoptosis rate（7.77%±2.57%vs21.23%±4.11%,t=-4.81,P〈0.01）was significantly reduced while the concentration of NO in the supernatant was significantly higher in the oridonin group which was pretreated with 5μmol/L oridonin before 500μmol/L palmitic acid as（191.33±13.01）μmol/L vs（114.67±17.01）μmol/L（t=6.20,P〈0.01）.Conclusion：Oridonin can inhibit the cell apoptosis in human umbilical vein endothelial cells induced by palmitic acid,and increase nitric oxide concentration.

关 键 词：冬凌草甲素 血管内皮细胞 棕榈酸 一氧化氮 

分 类 号：R587.1[医药卫生—内分泌]