骨髓间充质干细胞抑制外周血单核细胞向树突状细胞分化  

Bone Marrow Mesenchymal Stem Cells Inhibit Differentiation of Dendritic Cells from Monocytes

作　　者：徐志红[1] 李银萍[1] 

机构地区：[1]武汉大学基础医学院病理生理学系,湖北武汉430071

出　　处：《武汉大学学报（医学版）》2015年第3期354-358,共5页Medical Journal of Wuhan University

基　　金：国家自然科学基金资助项目(编号:81270411)

摘　　要：目的:探讨骨髓间充质干细胞(MSC)对单核细胞分化为树突状细胞(DC)的影响。方法:将分离培养的MSC与单核细胞共培养,加入DC分化诱导因子GM-CSF和IL-4(MSC+-DC),并以培养分化体系中无MSC存在的作为阳性对照(MSC--DC)。6d后收集悬浮细胞并用TNF-α诱导成熟48h。分别用流式细胞术、混合淋巴细胞反应及扫描电子显微镜检测细胞的表型、免疫功能及细胞形态。结果:同阳性对照DC相比,与MSC共培养得到的细胞上DC相关分子的表达明显较低,包括CD80,CD86,CD1a,CD83;而CD14分子则持续性高表达;与MSC共培养的细胞刺激异基因T淋巴细胞增殖的能力亦显著降低。形态学上,细胞胞体小,胞质突起不明显甚至缺如。结论:MSC抑制单核细胞向DC分化和成熟。Objective：To study the effects of bone marrow mesenchymal stem cells（MSCs）on the differentiation of DCs derived from monocytes.Methods：Monocytes were cultured with human MSCs in the presence of GM-CSF and IL-4.On day 6,cells in suspension were collected and TNF-αwas added to induce maturation of DCs for 48 hours.Phenotype,allogeneic stimulatory capacity and morphology of obtained cells were analyzed through cytometry,mixed lymphocyte reaction and scanning electronic microscopy.Results：Phenotypically,these cells expressed much lower levels of DC-related molecules,such as CD1 a,CD86,CD80 and CD83,whereas they expressed persistently high level of CD14.Functionally,their capacity to stimulate allogeneic T cells was significantly inhibited.Accordingly,these cells expressed rarely long and large cytoplasmic dendrites.Conclusion：MSCs inhibit the differentiation of monocytes into DCs.

关 键 词：骨髓间充质干细胞 树突状细胞 单核细胞 分化 

分 类 号：R392[医药卫生—免疫学]