壮药汗衣台对鸭乙型肝炎的治疗作用  

Therapeutic effect of Zhuang herb Hanyitai on duck hepatitis B

作　　者：廖丹[1] 马静[1] 段雪琳[1] 

机构地区：[1]广西中医药大学基础医学院生理教研室,广西壮族自治区南宁市530001

出　　处：《世界华人消化杂志》2015年第9期1395-1401,共7页World Chinese Journal of Digestology

基　　金：广西自然科学基金资助项目;No.2014GXNSFAA118211~~

摘　　要：目的:探讨壮药汗衣台对鸭乙型肝炎病毒(duck hepatitis B virus,DHBV)感染诱导的鸭乙型肝炎模型中肝功能、病毒复制、免疫调控及肝组织炎症方面的作用.方法:本研究采用DHBV阳性血清腹腔注射感染广西1日龄麻鸭诱导鸭乙型肝炎模型.于用药前(T0),用药7 d(T7),14 d(T14)及停药3d(P3)于颈静脉抽血,分别检测鸭血清中的谷丙转氨酶(alanine aminotransferase,ALT)、谷草转氨酶(aspartate aminotransferase,AST)、白介素-2(interleukin-2,IL-2)、DHBV DNA及肝组织病理.结果:模型组感染病毒1-2 wk,鸭血清ALT、AST与IL-2水平明显升高(P=0.028,0.036;P=0.005,0.04;P=0.045),病毒载量表达平稳,鸭肝组织病理检测显示重度脂肪样变性;拉米组治疗1-2 wk,鸭血清ALT、IL-2水平明显下降(P=0.001,0.042;P=0.023),但对AST水平无明显影响,病毒载量被显著抑制,停药3 d无反跳(P=0.034;P=0.007;P=0.013),肝组织病理提示中度以上脂肪变性;汗高组治疗1-2 wk,鸭血清ALT、AST和IL-2水平均明显降低(P=0.047,0.035;P=0.007,0.003;P=0.026,0.049),对病毒的抑制疗效与拉米组相似(P=0.025;P=0.012;P=0.011),肝组织病理显示中度脂肪变性;汗常组治疗1-2 wk,鸭血清ALT、AST及IL-2水平均明显降低(P=0.015;P=0.038;P=0.024,0.004),但在T14时,ALT、AST水平均出现回升,病毒载量呈缓慢下降趋势,肝组织病理提示中度以上脂肪变性.结论:汗衣台剂量依赖性的降低转氨酶、抑制DHBV DNA复制、减少IL-2分泌,从而保护肝细胞、减轻肝脂肪变性、降低免疫过强所导致的急性肝损伤.AIM：To investigate the therapeutic effect of Zhuang herb Hanyitai on liver function,viral replication,immune regulation and liver inflammation in a duck model infected by duck hepatitis B virus（DHBV）.METHODS：Duck hepatitis B was induced by intraperitoneal injection of DHBV positive serum into one-day-old Guangxi sheldrakes.On the day before treatment（TO）,7 d（T7）and 14 d（T14） during treatment,and 3d（P3） after treatment,jugular blood and liver tissue samples were collected to detect aspartate aminotransferase（ALT）,alanine aminotransferase（AST）,interleukin-2（IL-2）,DHBV DNA,and liver pathology.RESULTS：In the model group,ALT,AST and IL-2 levels were significantly increased after DHBV infection for one week and two weeks（P = 0.028,0.036;P = 0.005,0.04;P =0.045）,the amount of virus was relatively stable,and severe fatty degeneration was observed in liver tissues.In the lamivudine group,ALT and IL-2 levels were significantly decreased after treatment for one week and two weeks（P = 0.001,0.042;P = 0.023）,AST level showed no significant changes,the replication of virus was significantly inhibited（P = 0.034;0.007）,without rebounding after medication withdrawal for three days（P = 0.013）,and liver tissue pathology showed moderate liver steatosis.In the high dose Hanyitai group,ALT,AST,and IL-2 levels were significantly reduced after treatment for one week and two weeks（P = 0.047,0.035;P = 0.007,0.003;P = 0.026,0.049）,the replication of virus was inhibited significantly as the lamivudine group（P =0.025;P = 0.012;P = 0.011）,and liver tissue pathology displayed moderate liver steatosis.In the usual dose Hanyitai group,ALT,AST,and IL-2 levels were significantly reduced after treatment for one week and two weeks（P = 0.015;P = 0.038;P = 0.024,0.004）,but at T14,ALT and AST level rebounded,the replication of virus was inhibited moderately,and moderate or severe steatosis was noted.CONCLUSION：Hanyitai can reduce transaminase levels,inhibit DHBV DNA replication,and red

关 键 词：汗衣台 转氨酶 免疫应答 鸭乙型肝炎病毒 体内 

分 类 号：R29[医药卫生—民族医学]