原花青素抗氧化应激效应对大鼠肢体缺血再灌注后肠黏膜的保护作用  

Anti-oxidative stress effect of proanthocyanidin on intestinal mucosa of rats with limb ischemia-reperfusion injury

作　　者：宋强[1] 罗兰[1] 杨拯[2] 卓睿[1] 李禹呈[1] 陈勇[1] 唐丽娟[1] 黄倩[3] 计震华 杨德晶 

机构地区：[1]成都医学院临床医学院,四川省成都市610500 [2]成都医学院基础医学实验教学中心,四川省成都市610500 [3]成都医学院检验医学院,四川省成都市610500

出　　处：《世界华人消化杂志》2015年第9期1454-1459,共6页World Chinese Journal of Digestology

基　　金：成都医学院大学生创新实验基金资助项目;No.CX201220~~

摘　　要：目的:探究原花青素(proanthocyanidin,PC)抗氧化功能对大鼠肢体缺血再灌注(limb ischemia-reperfusion,LI/R)损伤后肠道的保护作用及可能的机制.方法:健康成年Sprague-Dawley♂大鼠21只,随机分为假手术组(n=7),缺血再灌注组(n=7),PC组(n=7);PC组给予PC 100 mg/(kg?d)预处理,其余两组灌胃等量色拉油,连续给药7 d.采用止血带捆绑大鼠左后肢复制缺血3 h后再灌注18 h大鼠LI/R模型,随后打开腹腔,分离腹主动脉取血离心,测定血清超氧化物歧化酶(superoxide dismutase,SOD)活力值和丙二醛(malondialdehyde,MDA)含量,取回肠组织进行苏木精-伊红(H E)染色,同时制备肠组织匀浆测定分泌型免疫球蛋白A(secretory immunoglobulin A,s Ig A)的含量.结果:与假手术组比较,LI/R后,血清SOD活力降低(P<0.01),MDA含量增多(P<0.01),大鼠肠黏膜损伤加重(P<0.01),且肠组织中s Ig A含量明显减少(P<0.01);与LI/R组相比,PC预处理后,大鼠血清SOD活力提高(P<0.05),MDA的含量明显减少(P<0.01),肠黏膜损伤减轻(P<0.01),肠组织中s Ig A含量上升(P<0.05).结论:PC可通过提高血清SOD活力,减少MDA含量,增加肠组织中s Ig A含量来减轻肠黏膜损伤,进而减轻LI/R后大鼠肠道损伤.AIM：To investigate the protective effect of proanthocyanidin（PC） on the intestinal mucosa of rats with limb ischemia-reperfusion（LI/R） and to explore the possible mechanism.METHODS：Twenty-one male adult SpragueDawley rats were randomly divided into three groups（n = 7）：sham operation group,LI/R group and PC group.The PC group received100 mg/（kg·d） PC via oral gavage for 7 d before LI/R.The sham operation and LI/R groups were given the same dose of salad oil each day for 7 d.A tourniquet was used to tie the rat left hind limb to induce LI/R injury in rats（ischemia 3 h and reperfusion18 h）.Following reperfusion,the abdominal cavity was opened and blood samples were taken from the abdominal aorta to determine serum superoxide dismutase（SOD） and malondialdehyde（MDA） levels.Segments of the ileum were quickly resected and equally separated into two parts to conduct hematoxylin eosin（HE） staining and determine tissue secretory immunoglobulin A（sIgA） levels,respectively.RESULTS：Compared with the sham operation group,serum SOD activity was significantly decreased（P〈0.01）,MDA levels were significantly increased（P〈 0.01）,mucosal injury was significantly aggravated in light microscopic evaluations（P〈0.01） and tissue sIgA（P〈0.01） was significantly reduced after LI/R.Compared with the LI/R group,pretreatment with PC significantly enhanced serum SOD activity（P〈0.05）,decreased MDA levels（P〈0.01）,mitigated intestinal mucosal injury（P〈0.01） and increased the tissue sIgA contents（P〈0.05）.CONCLUSION：PC protects the intestinal mucosa by elevating serum SOD activity,reducing MDA levels and increasing tissue sIgA contents in rats after LI/R injury.

关 键 词：原花青素 肢体缺血再灌注 抗氧化 

分 类 号：R285.5[医药卫生—中药学]